Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen E, Denmark.
Department of Ophthalmology, Copenhagen University Hospital, Glostrup, Denmark.
Acta Ophthalmol. 2024 Nov;102(7):773-778. doi: 10.1111/aos.16684. Epub 2024 Mar 30.
To compare the effect of the ocular antiseptic treatments 0.05% chlorhexidine, 5% povidone-iodine (PI) and 5% betadine on cell viability and mucin secretion of primary cultured human goblet cells (GCs).
GC viability was analysed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Expression of mucin was visualised by immunohistochemical MUC5AC staining.
PI and betadine significantly reduced GC survival compared to the control (mean cell survival 23 ± 6% and 23 ± 7%, respectively, p < 0.05), whereas chlorhexidine did not significantly affect GC viability (mean cell survival: 78 ± 17%), as measured by the LDH assay. Similar results were obtained from the MTT assay, where PI and betadine caused a significant loss of GCs (mean cell survival: 26 ± 12% and 26 ± 13%, respectively, p < 0.05). Chlorhexidine did not significantly alter GC survival compared to the control (mean cell survival: 79 ± 8%). PI and betadine caused a dispersion of mucin secretion, which chlorhexidine did not.
The most used antiseptic treatments, PI and betadine, applied prior to ocular surgery are significantly more cytotoxic to conjunctival GCs than chlorhexidine treatment.
比较眼部抗菌处理 0.05%洗必泰、5%聚维酮碘（PI）和 5%聚维酮碘对原代培养人杯状细胞（GC）活力和粘蛋白分泌的影响。
采用乳酸脱氢酶（LDH）和噻唑蓝（MTT）比色法分析 GC 活力。用免疫组化 MUC5AC 染色法观察粘蛋白的表达。
PI 和 betadine 与对照组相比，明显降低 GC 的存活率（细胞存活率分别为 23±6%和 23±7%，p<0.05），而洗必泰对 GC 活力没有明显影响（LDH 检测细胞存活率为 78±17%）。MTT 检测也得到了类似的结果，PI 和 betadine 导致 GC 大量死亡（细胞存活率分别为 26±12%和 26±13%，p<0.05）。与对照组相比，洗必泰对 GC 存活率无明显影响（细胞存活率为 79±8%）。PI 和 betadine 导致粘蛋白分泌分散，而洗必泰则没有。
在眼部手术前应用的最常用的抗菌处理剂 PI 和 betadine 对结膜 GC 的细胞毒性明显高于洗必泰处理。
Zotero 插件