Deamant F D, Iannaccone P M
J Natl Cancer Inst. 1985 Jan;74(1):145-50.
Subcutaneous fibrosarcomas were induced chemically in mosaic mice heterozygous at the Pgk-1 locus on the X-chromosome. The normal tissues of these animals displayed two electrophoretically distinct isozymes of phosphoglycerate kinase-1-[(PGK-1) EC 2.7.2.3]. The tumors were carefully dissected and analyzed with respect to PGK-1 isozymes. A combination method of multiple sampling of the primary tumor and establishment of tissue culture cell lines from the tumor was utilized. A total of 82 samples was studied from 10 tumors developed in 9 mice. Twenty cell lines were established from 9 tumors; 6 of the tumors were shown to contain neoplastic cells of exclusively PGK-1B activity, 3 of the tumors were shown to contain neoplastic cells of exclusively PGK-1A activity, and 1 tumor contained neoplastic cells with both PGK-1A and PGK-1B activities. The tumor with both A and B activities was hourglass shaped, and the pattern of activity from multiple samples of this tumor suggests that it was formed by the coalescence of a PGK-1A and a PGK-1B tumor. The data presented are concordant with results from chimeric animals and spontaneous tumors in humans and indicate that these tumors display the behavior of clonal growths.
在X染色体上的磷酸甘油酸激酶-1（Pgk-1）基因座杂合的嵌合小鼠中，通过化学方法诱导产生皮下纤维肉瘤。这些动物的正常组织显示出两种电泳性质不同的磷酸甘油酸激酶-1同工酶-[(PGK-1) EC 2.7.2.3]。对肿瘤进行仔细解剖，并分析其PGK-1同工酶。采用了对原发性肿瘤进行多次取样并从肿瘤中建立组织培养细胞系的联合方法。对9只小鼠中发生的10个肿瘤的总共82个样本进行了研究。从9个肿瘤中建立了20个细胞系；其中6个肿瘤显示仅含有具有PGK-1B活性的肿瘤细胞，3个肿瘤显示仅含有具有PGK-1A活性的肿瘤细胞，1个肿瘤含有同时具有PGK-1A和PGK-1B活性的肿瘤细胞。同时具有A和B活性的肿瘤呈沙漏形，对该肿瘤多个样本的活性模式分析表明，它是由一个PGK-1A肿瘤和一个PGK-1B肿瘤合并形成的。所呈现的数据与嵌合动物和人类自发肿瘤的结果一致，表明这些肿瘤表现出克隆生长的行为。
