Department of Pharmacy, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
Department of General Practice, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
Fitoterapia. 2024 Jun;175:105930. doi: 10.1016/j.fitote.2024.105930. Epub 2024 Mar 28.
Two new quinoline alkaloids with an α, β-unsaturated amide side chain, xylarinines A and B (1 and 2), were isolated from the ethyl acetate extracts of Xylaria longipes solid fermentation. The structures of these were primarily determined though NMR and HRESIMS data analysis. The absolute configuration of compound 1 was assigned using experimental and calculated ECD data. The neuroprotective effects of compounds 1 and 2 against glutamate-induced damage in PC12 cells were evaluated in vitro bioassay. The results demonstrated that both compounds significantly improved cell viability, inhibited apoptosis, decreased malondialdehyde (MDA) levels, increased superoxide dismutase (SOD) and glutathione (GSH) levels, and reduced intracellular reactive oxygen species (ROS) accumulation. These findings suggested that these mechanisms contribute to the neuroprotective effects of the compounds.
从长拟青霉固态发酵的乙酸乙酯提取物中分离得到两个具有α,β-不饱和酰胺侧链的新喹啉生物碱：木霉素 A 和 B（1 和 2）。通过 NMR 和 HRESIMS 数据分析初步确定了这些结构。通过实验和计算的 ECD 数据确定了化合物 1 的绝对构型。在体外生物测定中评估了化合物 1 和 2 对谷氨酸诱导的 PC12 细胞损伤的神经保护作用。结果表明，这两种化合物均能显著提高细胞活力，抑制细胞凋亡，降低丙二醛（MDA）水平，增加超氧化物歧化酶（SOD）和谷胱甘肽（GSH）水平，减少细胞内活性氧（ROS）的积累。这些发现表明这些机制有助于化合物的神经保护作用。
