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本文引用的文献

1

Circular differential scattering can be an important part of the circular dichroism of macromolecules.

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利用圆偏振光对旋光生物结构进行成像。

Imaging of optically active biological structures by use of circularly polarized light.

作者信息

Keller D, Bustamante C, Maestre M F, Tinoco I

出版信息

Proc Natl Acad Sci U S A. 1985 Jan;82(2):401-5. doi: 10.1073/pnas.82.2.401.

DOI:10.1073/pnas.82.2.401

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397046/

Abstract

If an optically active (chiral) sample is placed in a microscope and illuminated with circularly polarized light, an image can be formed that is related to the circular dichroism of each feature of the sample. A theoretical investigation has been done for the circular differential image obtained by subtracting the images formed under right- and left-circularly polarized light. Two types of differential images are possible: (i) dark-field images formed from light reflected or scattered by the sample and (ii) bright-field images formed from light transmitted through the sample. The sign and magnitude of each feature in a circular differential image strongly depend on the structure of the sample. The dark-field circular differential images are most sensitive to large features with dimensions similar to the wavelength of illumination whereas the bright-field images are most sensitive to the short-range molecular order. Applications of circular differential imaging may include clinical fingerprinting of normal and transformed cells and structural analysis of individual cellular components.

摘要

如果将一个旋光性（手性）样品置于显微镜下并用圆偏振光照射，就可以形成与样品各特征的圆二色性相关的图像。对于通过减去在右旋和左旋圆偏振光下形成的图像而获得的圆差分图像进行了理论研究。可能有两种类型的差分图像：（i）由样品反射或散射的光形成的暗场图像，以及（ii）由透过样品的光形成的亮场图像。圆差分图像中每个特征的符号和大小强烈依赖于样品的结构。暗场圆差分图像对尺寸与照明波长相似的大特征最为敏感，而亮场图像对短程分子有序最为敏感。圆差分成像的应用可能包括正常细胞和转化细胞的临床指纹识别以及单个细胞成分的结构分析。