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The differential scattering of circularly polarized light by chloroplasts and evaluation of their true circular dichroism.叶绿体对圆偏振光的微分散射及其真实圆二色性的评估。
Biochem J. 1974 Aug;142(2):193-201. doi: 10.1042/bj1420193.
2
Differential polarization imaging. V. Numerical aperture effects and the contribution of preferential scattering and absorption to the circular dichroism images.差分偏振成像。V. 数值孔径效应以及优先散射和吸收对圆二色性图像的贡献。
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Differential light-scattering of granal and agranal chloroplasts and their fragments.基粒叶绿体和基质叶绿体及其片段的微分光散射
Biochem J. 1978 Aug 15;174(2):647-51. doi: 10.1042/bj1740647.
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Experimental differential light-scattering correction to the circular dichroism of bacteriophage T2.噬菌体T2圆二色性的实验性微分光散射校正
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Helically organized macroaggregates of pigment-protein complexes in chloroplasts: evidence from circular intensity differential scattering.叶绿体中色素 - 蛋白质复合物的螺旋状排列大聚集体：来自圆二色性散射的证据
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[Interaction of the photosystem 1 reaction center with antenna chlorophyll in a pigment-protein complex isolated from pea chloroplasts].[从豌豆叶绿体分离的色素-蛋白复合物中光系统1反应中心与天线叶绿素的相互作用]
Mol Biol (Mosk). 1985 May-Jun;19(3):841-50.
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Circular differential scattering can be an important part of the circular dichroism of macromolecules.圆二色性散射可能是大分子圆二色性的一个重要组成部分。
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Evidence that circularly dichroic chlorophyll forms a-682 and a-710 are oriented at right angles to the thylakoid membranes of whole chloroplasts, and that the circular dichroism is light-dependent.圆二色性叶绿素a-682和a-710与完整叶绿体的类囊体膜呈直角排列，且圆二色性依赖于光的证据。
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Circular dichroism analyses of membrane proteins: an examination of differential light scattering and absorption flattening effects in large membrane vesicles and membrane sheets.膜蛋白的圆二色性分析：对大膜泡和膜片层中差分光散射和吸收平坦化效应的研究。
Anal Biochem. 1984 Nov 1;142(2):317-28. doi: 10.1016/0003-2697(84)90471-8.

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Effect of circularly polarized light on germination, hypocotyl elongation and biomass production of arabidopsis and lettuce: Involvement of phytochrome B.圆偏振光对拟南芥和生菜种子萌发、下胚轴伸长及生物量生产的影响：光敏色素B的作用
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A simple method for correction of circular dichroism spectra obtained from membrane-containing samples.一种从含膜样品中获得的圆二色光谱的简单校正方法。
Biochemistry. 2012 Feb 7;51(5):1005-8. doi: 10.1021/bi300025c. Epub 2012 Jan 27.
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Organization of Chlorophyll a in the Light-Harvesting Chlorophyll a/b Protein Complex as Shown by Circular Dichroism : Liquid Crystal-Like Domains.圆二色性研究表明：叶绿素 a 在捕光叶绿素 a/b 蛋白复合物中的组织形式类似于液晶结构域。
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5
Evidence that circularly dichroic chlorophyll forms a-682 and a-710 are oriented at right angles to the thylakoid membranes of whole chloroplasts, and that the circular dichroism is light-dependent.圆二色性叶绿素a-682和a-710与完整叶绿体的类囊体膜呈直角排列，且圆二色性依赖于光的证据。
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7
Differential light-scattering of granal and agranal chloroplasts and their fragments.基粒叶绿体和基质叶绿体及其片段的微分光散射
Biochem J. 1978 Aug 15;174(2):647-51. doi: 10.1042/bj1740647.
8
Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes.叶绿体类囊体膜中518纳米处的光依赖性吸收和选择性散射变化。
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本文引用的文献

1
The flattening of the absorption spectrum of suspensions, as compared to that of solutions.与溶液相比，悬浮液吸收光谱的扁平化。
Biochim Biophys Acta. 1956 Jan;19(1):1-12. doi: 10.1016/0006-3002(56)90380-8.
2
The molecular structure of photosynthetic lamellar systems.光合片层系统的分子结构。
Brookhaven Symp Biol. 1966;19:328-40.
3
Chlorophyll-chlorophyll interactions.叶绿素-叶绿素相互作用
Brookhaven Symp Biol. 1966;19:303-18.
4
Optical rotary dispersion and circular dichroism studies on mitochondria: correlation of ultrastructure and metabolic state with molecular conformational changes.线粒体的旋光色散和圆二色性研究：超微结构和代谢状态与分子构象变化的相关性
Arch Biochem Biophys. 1968 Dec;128(3):790-801. doi: 10.1016/0003-9861(68)90087-8.
5
Optical rotation of mitochondria and its relation to conformation.线粒体的旋光性及其与构象的关系。
Biochem Biophys Res Commun. 1968 Mar 12;30(5):476-82. doi: 10.1016/0006-291x(68)90076-4.
6
Optical activity of membrane suspensions: calculation of artifacts by Mie scattering theory.膜悬浮液的旋光性：基于米氏散射理论的假象计算
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2365-9. doi: 10.1073/pnas.68.10.2365.
7
Optical activity of biological membranes: scattering effects and protein conformation.生物膜的旋光性：散射效应与蛋白质构象
Proc Natl Acad Sci U S A. 1970 Jul;66(3):793-8. doi: 10.1073/pnas.66.3.793.
8
Differential scatter of left and right circularly polarized light by optically active particulate systems.旋光性颗粒系统对左旋和右旋圆偏振光的差异散射。
Proc Natl Acad Sci U S A. 1970 Apr;65(4):845-52. doi: 10.1073/pnas.65.4.845.
9
Circular dichroism of biological membranes. I. Mitochondria and red blood cell ghosts.生物膜的圆二色性。I. 线粒体和红细胞影
Biochim Biophys Acta. 1971 Aug 13;241(2):600-12. doi: 10.1016/0005-2736(71)90058-7.
10
Are specific chlorophyll-protein complexes required for photosynthesis?光合作用是否需要特定的叶绿素 - 蛋白质复合物？
Biochim Biophys Acta. 1971 Jun 15;234(3):330-4. doi: 10.1016/0005-2728(71)90199-x.

叶绿体对圆偏振光的微分散射及其真实圆二色性的评估。

The differential scattering of circularly polarized light by chloroplasts and evaluation of their true circular dichroism.

作者信息

Gregory R P, Raps S

出版信息

Biochem J. 1974 Aug;142(2):193-201. doi: 10.1042/bj1420193.

DOI:10.1042/bj1420193
PMID:4441376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168268/
Abstract

Chloroplasts isolated from pea leaves display an intense circular dichroism in the range 600 to 720nm. Circularly polarized light is also differentially scattered by chloroplasts, and this effect can be confused with circular dichroism. By using an instrumental modification it was possible to distinguish, and record separately, the ellipticities of the transmitted light (circular dichroism) and of the scattered light in the same c.d. instrument. By means of a light-scattering apparatus, the intensity of unpolarized light scattered by chloroplasts was measured as a function of wavelength and of angle. This measurement allowed the aforementioned ellipticities to be corrected for mutual interference. At a concentration of 4mug of chlorophyll/ml (the optimum practical concentration of chloroplasts at which there was no significant interaction of scattering and absorption effects) spectra of true circular dichroism (circular differential absorption) and circular differential scattering were obtained. The former showed maxima, positive at 688nm and negative at 676nm, with an intensity Deltatheta=8.3m degrees .litre.(mg of chlorophyll)(-1).cm(-1). The latter had a maximum at 683nm with an intensity of +47m degrees with respect to the solvent baseline; this value is independent of the concentration of chloroplasts in dilute suspensions. It is suggested that the intense circular dichroism of chloroplasts reflects specific chlorophyll-chlorophyll interactions in the light-harvesting pigment. The advantages of this method for determining the c.d. of scattering suspensions over those of other investigators are discussed.

摘要

从豌豆叶片中分离出的叶绿体在600至720纳米范围内呈现出强烈的圆二色性。圆偏振光也会被叶绿体以不同方式散射，这种效应可能会与圆二色性混淆。通过对仪器进行改进，得以在同一圆二色性仪器中区分并分别记录透射光（圆二色性）和散射光的椭圆率。借助光散射装置，测量了叶绿体散射的非偏振光强度随波长和角度的变化。该测量使得上述椭圆率能够因相互干扰而得到校正。在叶绿素浓度为4微克/毫升时（这是叶绿体的最佳实际浓度，此时散射和吸收效应无显著相互作用），获得了真实圆二色性（圆差分吸收）和圆差分散射的光谱。前者在688纳米处出现正的最大值，在676纳米处出现负的最大值，强度Δθ = 8.3毫度·升·（毫克叶绿素）⁻¹·厘米⁻¹。后者在683纳米处有一个最大值，相对于溶剂基线强度为 +47毫度；该值与稀悬浮液中叶绿体的浓度无关。有人认为叶绿体强烈的圆二色性反映了光捕获色素中特定的叶绿素 - 叶绿素相互作用。讨论了这种测定散射悬浮液圆二色性的方法相对于其他研究者方法的优势。