探讨氟虫腈抗性与牛蜱（硬蜱属）感染边缘无浆体之间的关系。

Exploring the relationship between flumethrin resistance and Anaplasma marginale infection in Rhipicephalus (Boophilus) microplus ticks of cattle.

机构信息

Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari, Gandhinagar, 396 450, India.

Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari, Gandhinagar, 396 450, India.

出版信息

Trop Anim Health Prod. 2024 Apr 1;56(3):113. doi: 10.1007/s11250-024-03955-7.

DOI:10.1007/s11250-024-03955-7

PMID:38557923

Abstract

The study explores the relationship between flumethrin resistance and Anaplasma marginale infection in Rhipicephalus (Boophilus) microplus of cattle in South Gujarat, India. Adult Immersion Test (AIT) was used to assess flumethrin resistance and polymerase chain reaction (PCR) to confirm A. marginale infection. Species-specific PCR resulted in the amplification of 576 bp of msp5 gene of A. marginale in 17.69% (49/277) groups of ticks, and subsequent digestion with EcoRI cleaved it into two distinct segments. Navsari district, noted level Ι resistance [resistance factors (RF) = 1.78-3.34], and A. marginale prevalence was 16.67, 15.38, 23.08, 15.38, and 11.76% in Navsari, Jalalpore, Gandevi, Chikhli, and Vansda sub-districts, respectively. Similarly, Vyara and Dolvan sub-districts of Tapi observed level Ι resistance (RF = 1-3.63), with A. marginale positivity of 21.43 and 22.22%, while Valod and Songhad demonstrated susceptibility, with 14.29 and 12.50% of A. marginale, respectively. Moving to Surat, the Mahuva, Bardoli, Mandvi, Palsana, and Kamrej sub-districts observed the level Ι resistance (RF = 1.94-2.89), coupled with 14.29, 17.65, 20, 20, and 21.43% of A. marginale, respectively. Lastly, in Valsad district, Dharampur, Kaparada, Valsad, and Umbergaon noted level Ι resistance (RF = 1.67-1.81), and corresponding A. marginale positivity rates of 18.18, 19.23, 15.00, and 20.00%. The scatter plot unveiled a significant moderate positive correlation between RF and A. marginale positivity% (p = 0.0362), characterized by a Pearson correlation coefficient (r) of 0.4963. The covariance (1.1814) highlighted fluctuations, while the coefficient of determination (r) (0.2463) clarified that 24.63% of the variability in A. marginale positivity% could be attributed to the RF.

摘要

本研究旨在探讨印度古吉拉特邦南部的牛蜱（Boophilus）微小种对氟虫腈的抗药性与边缘无形体感染之间的关系。采用成虫浸浴试验（Adult Immersion Test，AIT）评估氟虫腈的抗药性，聚合酶链反应（polymerase chain reaction，PCR）确认边缘无形体的感染。种特异性 PCR 扩增出 17.69%（49/277）的边缘无形体 msp5 基因 576 bp，随后用 EcoRI 酶切将其分为两个不同的片段。纳瓦萨里地区表现出高度的抗性[抗性系数（Resistance Factors，RF）=1.78-3.34]，边缘无形体的流行率分别为纳瓦萨里、贾拉普尔、甘迪维、奇克利和万萨达等分区的 16.67%、15.38%、23.08%、15.38%和 11.76%。同样，塔皮的维亚拉和多尔万分区也观察到高度的抗性（RF=1-3.63），边缘无形体的阳性率分别为 21.43%和 22.22%，而瓦尔奥和松加德则表现出敏感性，边缘无形体的阳性率分别为 14.29%和 12.50%。在苏拉特，马胡瓦、巴尔多利、曼德维、帕尔萨纳、卡姆雷杰等分区观察到高度的抗性（RF=1.94-2.89），边缘无形体的阳性率分别为 14.29%、17.65%、20%、20%和 21.43%。最后，在瓦尔萨德地区，达尔普尔、卡帕拉达、瓦尔萨德和乌姆贝加翁分区观察到高度的抗性（RF=1.67-1.81），边缘无形体的阳性率分别为 18.18%、19.23%、15.00%和 20.00%。散点图揭示了 RF 与边缘无形体阳性率之间存在显著的中度正相关（p=0.0362），皮尔逊相关系数（r）为 0.4963。协方差（1.1814）突出了波动，而决定系数（r）（0.2463）表明，边缘无形体阳性率的 24.63%可以归因于 RF。