Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Skin Res Technol. 2024 Apr;30(4):e13685. doi: 10.1111/srt.13685.
Wound healing has evolved in recent years, resulting in diverse therapeutic options.
This study evaluated the effects of the somatic antigen of the hydatid cyst protoscolex on wound healing in mice with full-thickness skin wounds.
Fifty-four adult mice, weighing 25 ± 5 g and approximately 60 days old, were divided into three groups (A, B, and C), each further divided into three subgroups. Subgroups A1, A2, and A3 were assigned negative controls. B1, B2, and B3 received hydatid cyst somatic antigen tests at 10 µg/SC, whereas C1, C2, and C3 received somatic antigen tests at 20 µg/SC. Under general anesthesia, a wound biopsy puncture of 9.8 mm in diameter was performed on the mice's back and spine. In the experimental group, antigen and alum adjuvant were administered subcutaneously around the wound, while the control group received Phosphate-Buffered Saline (PBS). Using digital images, a geometric assessment was conducted on days 0, 1, 3, 6, 9, 12, 15, 18, and 21 post-wounding. The obtained images were analyzed by Image J software and after analyzing the data by SPSS software.
A significant difference in terms of epithelization was observed in the antigen treatment group with a dose of 20 µg on days 3 and 6 (P < 0.05). Furthermore, the 20 µg antigen group was significantly higher than the 10 µg antigen group in terms of this factor on day 3 (P < 0.05). Skin samples were taken from all wounds on days 3, 10 and 21 for microscopic evaluation. Regarding epithelization, on day 10, a significant difference was observed in the treatment group with a concentration of 10 µg with the control group and the treatment group with a concentration of 20 µg (P < 0.05).
Based on the results of the present study, it can be concluded that somatic antigens of protoscolex hydatid cyst are dose-dependent and antigens with a dose of 20 µg by subcutaneous injection accelerate wound healing and epithelialization.
近年来，伤口愈合领域取得了进展，出现了多种治疗选择。
本研究评估了细粒棘球蚴原头蚴体抗原对全层皮肤伤口小鼠伤口愈合的影响。
将 54 只成年小鼠（体重 25±5g，约 60 日龄）分为三组（A、B 和 C），每组进一步分为 3 个亚组。亚组 A1、A2 和 A3 为阴性对照组。B1、B2 和 B3 接受 10μg/SC 的细粒棘球蚴原头蚴体抗原试验，而 C1、C2 和 C3 接受 20μg/SC 的原头蚴体抗原试验。在全身麻醉下，对小鼠背部和脊柱进行直径为 9.8mm 的伤口活检穿刺。在实验组中，抗原和明矾佐剂被皮下注射到伤口周围，而对照组则接受磷酸盐缓冲盐水（PBS）。在 0、1、3、6、9、12、15、18 和 21 天的伤口后，使用数字图像对伤口进行几何评估。使用 Image J 软件对获得的图像进行分析，然后使用 SPSS 软件对数据进行分析。
在剂量为 20μg 的抗原治疗组中，第 3 天和第 6 天的上皮化差异有统计学意义（P<0.05）。此外，在第 3 天，20μg 抗原组的上皮化显著高于 10μg 抗原组（P<0.05）。在第 3、10 和 21 天，从所有伤口采集皮肤样本进行显微镜评估。关于上皮化，在第 10 天，浓度为 10μg 的治疗组与对照组和浓度为 20μg 的治疗组之间存在显著差异（P<0.05）。
根据本研究结果，可以得出结论，细粒棘球蚴原头蚴体抗原具有剂量依赖性，20μg 剂量的抗原通过皮下注射可加速伤口愈合和上皮化。
