Ye Shasha, Lu Yi, Li Guo, Li Dongfang, Wu Yujin, Yao Yuan
College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311215, China.
Appl Biochem Biotechnol. 2024 Nov;196(11):7805-7815. doi: 10.1007/s12010-024-04921-7. Epub 2024 Apr 1.
Polyvinyl chloride (PVC) waste is a major environmental challenge. In this study, we found that a PVC-eating insect, Tenebrio molitor, could survive by consuming PVC as a dietary supplement. To understand the gut symbiotic community, metagenomic analysis was performed to reveal the biodiversity of a symbiotic community in the midgut of Tenebrio molitor. Among them, seven genera were enriched from the midgut of the insect under culture conditions with PVC as carbon source. A strain of Stenotrophomonas maltophilia was isolated from the midgut symbiotic community of the plastic-eating Tenebrio molitor. To unravel the functional gene for the biodegradation enzyme, we sequenced the whole genome of Stenotrophomonas maltophilia and found that orf00390, annotated as a hydrolase, was highly expressed in the PVC culture niche.
聚氯乙烯（PVC）废料是一项重大的环境挑战。在本研究中，我们发现一种以PVC为食的昆虫——黄粉虫，可以通过将PVC作为膳食补充剂来存活。为了解肠道共生群落，我们进行了宏基因组分析，以揭示黄粉虫中肠共生群落的生物多样性。其中，在以PVC作为碳源的培养条件下，有七个属从该昆虫的中肠中富集出来。从以塑料为食的黄粉虫的中肠共生群落中分离出了一株嗜麦芽窄食单胞菌。为了阐明生物降解酶的功能基因，我们对嗜麦芽窄食单胞菌的全基因组进行了测序，发现注释为水解酶的orf00390在PVC培养环境中高度表达。
