College of Horticulture, China Agricultural University, Beijing, 100193, China.
College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
J Integr Plant Biol. 2024 Jun;66(6):1106-1125. doi: 10.1111/jipb.13654. Epub 2024 Apr 1.
It is generally accepted that jasmonate-ZIM domain (JAZ) repressors act to mediate jasmonate (JA) signaling via CORONATINE-INSENSITIVE1 (COI1)-mediated degradation. Here, we report a cryptic signaling cascade where a JAZ repressor, FvJAZ12, mediates multiple signaling inputs via phosphorylation-modulated subcellular translocation rather than the COI1-mediated degradation mechanism in strawberry (Fragaria vesca). FvJAZ12 acts to regulate flavor metabolism and defense response, and was found to be the target of FvMPK6, a mitogen-activated protein kinase that is capable of responding to multiple signal stimuli. FvMPK6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues, thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2, which acts to control the expression of lipoxygenase 3 (FvLOX3), an important gene involved in JA biosynthesis and a diverse array of cellular metabolisms. Our data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.
人们普遍认为茉莉酸-ZIM 结构域（JAZ）抑制剂通过 CORONATINE-INSENSITIVE1（COI1）介导的降解来介导茉莉酸（JA）信号。在这里，我们报告了一个隐藏的信号级联，其中一个 JAZ 抑制剂 FvJAZ12 通过磷酸化调节的亚细胞易位介导多种信号输入，而不是草莓（ Fragaria vesca）中的 COI1 介导的降解机制。FvJAZ12 作用于调节风味代谢和防御反应，并且被发现是 FvMPK6 的靶标，FvMPK6 是一种能够响应多种信号刺激的丝裂原激活蛋白激酶。FvMPK6 在靠近 PY 残基的氨基酸残基 S179 和 T183 处磷酸化 FvJAZ12，从而减弱其核积累并解除其对 FvMYC2 的抑制，FvMYC2 作用于控制脂氧合酶 3（FvLOX3）的表达，FvLOX3 是参与 JA 生物合成和多种细胞代谢的重要基因。我们的数据揭示了 JA 信号的一个以前未报道的机制，并破译了一个信号级联，该级联将多种信号输入与果实特性发育联系起来。
