Fan Yuhan, Wang Yuchun, Tu Yiyi, Jiang Hong, Xu Ting, Wei Chaoling, Lv Wuyun
College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou, 311300, Zhejiang, China.
Zhejiang International Science and Technology Cooperation Base for Plant Germplasm Resources Conservation and Utilization, Zhejiang A&F University, Hangzhou, China, 311300.
Plant Cell Rep. 2025 Jul 13;44(8):174. doi: 10.1007/s00299-025-03563-1.
CsJAZ11 interacts with CsMYC2.2 in the nucleus and positively regulates disease resistance to Colletotrichum camelliae in tea plants. In the jasmonic acid signaling pathway, JASMONATE ZIM DOMAIN (JAZ) proteins act as repressors by inhibiting the activity of MYELOCYTOMATOSIS (MYC) transcription factors. However, the role of JAZ proteins in modulating tea plant resistance to Colletotrichum camelliae, a major causal pathogen of tea anthracnose, remains largely unexplored. In our previous study, yeast two-hybrid analysis revealed an interaction between CsJAZ11 and CsMYC2.2, a transcription factor that negatively regulates tea plant resistance to C. camelliae. In this study, bimolecular fluorescence complementation assays further confirmed that CsJAZ11 interacts with CsMYC2.2 in the nucleus. Co-immunoprecipitation assays also validated this interaction. To investigate the role of CsJAZ11 in regulating tea plant resistance to C. camelliae, transient silencing and overexpression of CsJAZ11 were performed in tea plant leaves. Notably, transient silencing of CsJAZ11 attenuates disease resistance to C. camelliae, while overexpression of CsJAZ11 enhanced disease resistance, suggesting that CsJAZ11 positively regulates tea plant resistance to C. camelliae. Surprisingly, qRT-PCR analysis showed that CsJAZ11 did not inhibit the expression of CsMYC2.2. In contrast, CsJAZ11 positively influenced the expression of CsRPM1, a MeJA-responsive CNL gene essential for disease resistance to C. camelliae in tea plants. Our findings indicate that CsJAZ11 positively regulates tea plant resistance to C. camelliae, potentially through the modulation of CsRPM1, an NBS-LRR-encoding gene. This study provides new insights into the role of JAZ proteins in regulating disease resistance in tea plants.
CsJAZ11在细胞核中与CsMYC2.2相互作用，并正向调控茶树对茶炭疽病菌（Colletotrichum camelliae）的抗病性。在茉莉酸信号通路中，茉莉酸ZIM结构域（JAZ）蛋白通过抑制髓细胞瘤（MYC）转录因子的活性发挥阻遏作用。然而，JAZ蛋白在调控茶树对茶炭疽病主要致病病原菌茶炭疽病菌的抗性方面的作用，在很大程度上仍未得到探索。在我们之前的研究中，酵母双杂交分析揭示了CsJAZ11与CsMYC2.2之间的相互作用，CsMYC2.2是一种负向调控茶树对茶炭疽病菌抗性的转录因子。在本研究中，双分子荧光互补分析进一步证实CsJAZ11在细胞核中与CsMYC2.2相互作用。免疫共沉淀分析也验证了这种相互作用。为了研究CsJAZ11在调控茶树对茶炭疽病菌抗性中的作用，在茶树叶片中对CsJAZ11进行了瞬时沉默和过表达。值得注意的是，CsJAZ11的瞬时沉默减弱了对茶炭疽病菌的抗病性，而CsJAZ11的过表达增强了抗病性，这表明CsJAZ11正向调控茶树对茶炭疽病菌的抗性。令人惊讶的是，qRT-PCR分析表明CsJAZ11并没有抑制CsMYC2.2的表达。相反，CsJAZ11正向影响CsRPM1的表达，CsRPM1是一个对茶树抗茶炭疽病菌至关重要的茉莉酸甲酯响应CNL基因。我们的研究结果表明，CsJAZ11可能通过调节编码NBS-LRR的基因CsRPM1，正向调控茶树对茶炭疽病菌的抗性。这项研究为JAZ蛋白在调控茶树抗病性中的作用提供了新的见解。
