仿生银纳米颗粒对斑马鱼胚胎的有益影响，包括一项基因表达研究。
Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study.
作者信息
R Sakthi Devi, Girigoswami Agnishwar, Meenakshi Shanmugaraja, Deepika Balasubramanian, Harini Karthick, Gowtham Pemula, Pallavi Pragya, Girigoswami Koyeli
机构信息
Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, India.
出版信息
ADMET DMPK. 2024 Jan 1;12(1):177-192. doi: 10.5599/admet.2102. eCollection 2024.
BACKGROUND AND PURPOSE
Many sectors use nanoparticles and dispose of them in the aquatic environment without deciding the fate of these particles.
EXPERIMENTAL APPROACH
To identify a benign species of nanoparticles which can cause minimum harm to the aquatic environment, a comparative study was done with chemically synthesized silver nanoparticles (AgNPs) and green tea mediated synthesis (GT/AgNP) in both in vitro using human alveolar cancer cell line (A549) and normal cell line (L132), and in in vivo with zebrafish embryos.
KEY RESULTS
The in vitro studies revealed that GT/AgNPs were less toxic to normal cells than cancer cells. The GT/AgNPs showed high biocompatibility for zebrafish embryos monitored microscopically for their developmental stages and by cumulative hatchability studies. The reduced hatchability found in the AgNPs-treated group was correlated by differential gene expression of zebrafish hatching enzymes (ZHE) (ZHE1 and ZHE2).
CONCLUSION
The results indicated that nanoparticles can affect the hatching of zebrafish embryos and elicit toxicity at the gene level.
背景与目的
许多行业使用纳米颗粒并将其排放到水生环境中，却未确定这些颗粒的最终归宿。
实验方法
为了确定对水生环境危害最小的良性纳米颗粒种类，使用人肺泡癌细胞系（A549）和正常细胞系（L132）在体外，以及使用斑马鱼胚胎在体内，对化学合成银纳米颗粒（AgNPs）和绿茶介导合成的纳米颗粒（GT/AgNP）进行了比较研究。
关键结果
体外研究表明，GT/AgNPs对正常细胞的毒性低于癌细胞。通过显微镜监测斑马鱼胚胎的发育阶段以及累积孵化率研究发现，GT/AgNPs对斑马鱼胚胎具有高生物相容性。在AgNPs处理组中发现的孵化率降低与斑马鱼孵化酶（ZHE）（ZHE1和ZHE2）的差异基因表达相关。
结论
结果表明，纳米颗粒可影响斑马鱼胚胎的孵化并在基因水平引发毒性。
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