Aavula Thabitha, Narasimman Vignesh, Ramachandran Saravanan, Murugan Radajurai, Ponnusamy Murugavel, Pazhani Gururaja Perumal, G Sivaleela
Native Medicine and Marine Pharmacology Laboratory, Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam 603 103, Tamil Nadu, India.
Department of Food Technology, M S Ramaiah University of Applied Sciences, Bangaluru, Karnataka, India.
Evid Based Complement Alternat Med. 2022 Jul 11;2022:3520336. doi: 10.1155/2022/3520336. eCollection 2022.
This study examines the antioxidant and teratogenic effects of two different type's methods of formulating agar from () using a zebrafish model. The agar was extracted using the aqueous extraction method and developed in two different formulations using separate procedures. Formulated agar1 (FA1) used a higher concentration of the ingredients while formulated agar 2 (FA2) had a lesser concentration. The two unique formulated agars (FAs) were studied using biochemical composition, Fourier infrared (FT-IR) spectroscopy, gas chromatography-mass spectroscopy (GC-MS), and scanning electron microscopy (SEM). The antioxidant activities of both FAs in vitro were shown to be significantly different ( < 0.05) at various concentrations (60-180 l/ml) in the study. The toxicity of the FAs was dose-dependent, with FA1 having the least teratogenic activity when compared to FA2. In comparison to FA2, FA1 was found to have higher antioxidant activity. At various concentrations (0.5, 0.25, and 0.125 g/ml), the teratogenic activity of two FAs was examined in zebrafish embryos (ZFE) at 24, 48, 72, and 96 hours post fertilization (hpf). Both FAs exhibit dose-dependent toxicity and increased antioxidant activity, and this can be utilized as an alternative for standard antioxidants, according to this study.
本研究使用斑马鱼模型，研究了两种不同类型的从（）制备琼脂的方法的抗氧化和致畸作用。琼脂采用水提法提取，并通过不同程序开发出两种不同配方。配方琼脂1（FA1）使用了较高浓度的成分，而配方琼脂2（FA2）的浓度较低。使用生化成分分析、傅里叶红外（FT-IR）光谱、气相色谱-质谱（GC-MS）和扫描电子显微镜（SEM）对这两种独特的配方琼脂（FAs）进行了研究。在该研究中，两种FAs在不同浓度（60-180μl/ml）下的体外抗氧化活性显示出显著差异（<0.05）。FAs的毒性呈剂量依赖性，与FA2相比，FA1的致畸活性最低。与FA2相比，发现FA1具有更高的抗氧化活性。在受精后24、48、72和96小时（hpf），在斑马鱼胚胎（ZFE）中检测了两种FAs在不同浓度（0.5、0.25和0.125g/ml）下的致畸活性。根据这项研究，两种FAs均表现出剂量依赖性毒性并具有增强的抗氧化活性，这可作为标准抗氧化剂的替代品。
