School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212000, China.
College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jun 5;314:124226. doi: 10.1016/j.saa.2024.124226. Epub 2024 Mar 29.
Organophosphorus pesticides play an important role as broad-spectrum inactivating herbicides in agriculture. Developing a method for rapid and efficient organophosphorus pesticides detection is still urgent due to the increasing concern on food safety. An organo-probe (ZDA), synthesized by purine hydrazone derivative and 2,2'-dipyridylamine derivative, was applied in sensitive recognition of Cu with detection limit of 300 nM. Mechanism study via density functional theory (DFT) and job's plot experiment revealed that ZDA and Cu ions form a 1:2 complex quenching the fluorescence emission. Moreover, this fluorescent complex ZDA-Cu was applicable for detecting glyphosate and glufosinate ammonium following fluorescence enhancement mechanism, with detection limits of 11.26 nM and 11.5 nM, respectively. Meanwhile, ZDA-Cu was effective and sensitive when it is used for pesticide detection, reaching the maximum value and stabilizing in 1 min. Finally, the ZDA-Cu probe could also be tolerated in cell assay environment, implying potential bio-application.
有机磷农药在农业中作为广谱灭生性除草剂起着重要作用。由于人们对食品安全越来越关注，因此开发一种快速、高效的有机磷农药检测方法仍然迫在眉睫。通过嘌呤腙衍生物和 2,2'-联吡啶胺衍生物合成的有机探针（ZDA），对 Cu 的检测限低至 300 nM，对其具有灵敏的识别作用。通过密度泛函理论（DFT）和 Job 实验研究表明，ZDA 与 Cu 离子形成 1:2 络合物，从而猝灭荧光发射。此外，该荧光配合物 ZDA-Cu 可用于检测草甘膦和草铵膦，基于荧光增强机制，其检测限分别为 11.26 nM 和 11.5 nM。同时，ZDA-Cu 用于农药检测时效果灵敏，在 1 分钟内达到最大值并稳定。最后，ZDA-Cu 探针在细胞检测环境中也具有耐受性，这意味着其具有潜在的生物应用。
