Research & Development Center for Eco-Material and Eco-Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730101, China.
Biosensors (Basel). 2023 Apr 28;13(5):510. doi: 10.3390/bios13050510.
A novel and sensitive fluorescent probe based on Cu-modulated polydihydroxyphenylalanine nanoparticles (PDOAs) has been developed for the detection of glyphosate pesticides. Compared to conventional instrumental analysis techniques, fluorometric methods have obtained good results in the field of agricultural residue detection. However, most of the fluorescent chemosensors reported still have some limitations, such as long response times, the high limit of detection, and complex synthetic procedures. In this paper, a novel and sensitive fluorescent probe based on Cu modulated polydihydroxyphenylalanine nanoparticles (PDOAs) has been developed for the detection of glyphosate pesticides. The fluorescence of PDOAs can be effectively quenched by Cu through the dynamic quenching process, which was confirmed by the time-resolved fluorescence lifetime analysis. In the presence of glyphosate, the fluorescence of the PDOAs-Cu system can be effectively recovered due to the higher affinity of glyphosate for Cu, and thus released the individual PDOAs. Due to the admirable properties such as high selectivity to glyphosate pesticide, "turn on" fluorescence response, and ultralow detection limit of 1.8 nM, the proposed method has been successfully applied for the determination of glyphosate in environmental water samples.
一种基于 Cu 调控聚二羟基苯丙氨酸纳米粒子（PDOAs）的新型灵敏荧光探针已被开发用于检测草甘膦农药。与传统的仪器分析技术相比，荧光法在农残检测领域取得了较好的效果。然而，大多数报道的荧光化学传感器仍然存在一些局限性，例如响应时间长、检测限高和合成步骤复杂。本文开发了一种基于 Cu 调控聚二羟基苯丙氨酸纳米粒子（PDOAs）的新型灵敏荧光探针，用于检测草甘膦农药。通过时间分辨荧光寿命分析证实，Cu 可以通过动态猝灭过程有效地猝灭 PDOAs 的荧光。在草甘膦存在的情况下，由于草甘膦对 Cu 具有更高的亲和力，PDOAs-Cu 体系的荧光可以得到有效恢复，从而释放出单个 PDOAs。由于对草甘膦农药具有高选择性、“开启”荧光响应和超低检测限（1.8 nM）等优良性能，该方法已成功应用于环境水样中草甘膦的测定。
