A study of chromosome content of Friend virus-induced mouse erythroleukemia cells (clone M2) via karyotype reconstruction.

Clone M2 of a Friend virus-induced mouse erythroleukemia cell line has been studied using G- and C-banding and Ag-staining. The modal chromosome number was 37. The population showed a remarkable karyotype stability and a similar chromosome content. Nullisomy and monosomy were recorded for 12 pairs of chromosomes. Determination of the origin of all 13 marker chromosomes made it possible to establish exact chromosome content of each cell. The generalized reconstructed karyotype of the cell line investigated was established by reconstructing cell karyotypes. This made it possible to demonstrate the retention of a mouse diploid chromosome set (40,XY), constant extra copies of chromosomes #2, #3, and #19, and for some cells, #9.