Dowjat K, Włodarska I
J Natl Cancer Inst. 1981 Jan;66(1):177-82.
The karyotype of murine lymphoblastic leukemia L1210 was studied with the use of the trypsin-Giemsa technique. The line was hypodiploid with a modal number of 38 chromosomes, including three biarmed chromosomes. All cells examined contained at least one copy of each normal chromosome with the exception of chromosome #1, in which a whole copy along with parts of the second copy was found in the marker chromsome's composition. In 65% of the cells, trisomy of chromosome #15 was observed. Nearly all cells contained only one X-chromosome. The 13 marker chromosomes that frequently occurred are described, and the presumed origin of seven of these chromosomes is described in detail. Comparison of the karyotype of murine L1210 leukemia with the karyotypes of other luekemias of the mouse showed the existence of similar chromosome aberrations. Among these aberrations, the trisomy of chromosome #15 and monosomy of the X-chromosome may be of special importance. The possible role of karyotypic changes in the genesis of the malignant phenotype of the cells is discussed.
运用胰蛋白酶 - 吉姆萨技术对小鼠淋巴细胞白血病L1210的核型进行了研究。该细胞系为亚二倍体，众数染色体数为38条，包括三条双臂染色体。除了1号染色体外，所有检测的细胞均含有每条正常染色体的至少一个拷贝，在标记染色体组成中发现1号染色体有一整条拷贝以及第二条拷贝的部分片段。在65%的细胞中观察到15号染色体三体。几乎所有细胞仅含有一条X染色体。描述了经常出现的13条标记染色体，并详细描述了其中7条染色体的推测起源。将小鼠L1210白血病的核型与小鼠其他白血病的核型进行比较，发现存在相似的染色体畸变。在这些畸变中，15号染色体三体和X染色体单体可能具有特殊重要性。讨论了核型变化在细胞恶性表型发生中的可能作用。
