Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Innovation Centre for Brewing & Fermentation, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Syst Appl Microbiol. 2024 May;47(2-3):126505. doi: 10.1016/j.syapm.2024.126505. Epub 2024 Mar 22.
The increase in studies on bee microbiomes is prompted by concerns over global pollinator declines. Bumble bees host core and non-core microbiota which may contribute to increased lifetime fitness. The presence of Fructobacillus in the gut microbiomes of bumble bee workers, or the replacement of core symbionts with Fructobacillus bacteria, has been considered a marker of dysbiosis. A phylogenomic analysis and functional genomic characterization of the genomes of 21 Fructobacillus isolates from bumble bees demonstrated that they represented four species, i.e. Fructobacillus cardui, Fructobacillus fructosus, Fructobacillus tropaeoli, and the novel species Fructobacillus evanidus sp. nov. Our results confirmed and substantiated the presence of two phylogenetically and functionally distinct Fructobacillus species clades that differ in genome size, percentage G + C content, the number of coding DNA sequences and metabolic characteristics. Clade 1 and clade 2 species differed in amino acid and, to a lesser extent, in carbohydrate metabolism, with F. evanidus and F. tropaeoli genomes featuring a higher number of complete metabolic pathways. While Fructobacillus genomes encoded genes that allow adhesion, biofilm formation, antibacterial activity and detoxification, other bacteria isolated from the bumble bee gut appeared better equipped to co-exist with the bumble bee host. The isolation and identification of multiple Fructobacillus species from several bumble bee gut samples in the present study also argued against a specific partnership between Fructobacillus species and their bumble bee hosts.
人们对全球传粉媒介减少的担忧促使有关蜜蜂微生物组的研究增多。熊蜂体内存在核心和非核心微生物群，这些微生物群可能有助于提高其终生适应能力。在熊蜂工蜂的肠道微生物群中存在果糖杆菌，或核心共生体被果糖杆菌取代，这被认为是失调的标志。对 21 株来自熊蜂的果糖杆菌的基因组进行了系统发育分析和功能基因组特征分析，结果表明它们代表了 4 个种，即蜡状果糖杆菌、果糖果糖杆菌、过路果糖杆菌和新种嗜酸果糖杆菌。我们的研究结果证实并证实了存在两个在系统发育和功能上有明显区别的果糖杆菌种系群，它们在基因组大小、G+C 含量百分比、编码 DNA 序列数量和代谢特征上存在差异。1 类和 2 类群在氨基酸代谢上存在差异，在碳水化合物代谢上的差异较小，而 F. evanidus 和 F. tropaeoli 基因组具有更多的完整代谢途径。虽然果糖杆菌基因组编码了允许黏附、生物膜形成、抗菌活性和解毒的基因，但从熊蜂肠道中分离出的其他细菌似乎更能与熊蜂宿主共存。在本研究中，从多个熊蜂肠道样本中分离和鉴定出多种果糖杆菌也表明果糖杆菌与它们的熊蜂宿主之间没有特定的伙伴关系。
