Chen Yi-Sheng, Wang Li-Ting, Lin Shih-Ting, Lee Yun-Shien, Chang Yu-Chung, Wu Hui-Chung, Liao Chia-Yu, Chen Wei-Hua, Deng Jin-Nan, Wang Yu-Hsuan
Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC.
Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC.
Int J Syst Evol Microbiol. 2022 Nov;72(11). doi: 10.1099/ijsem.0.005613.
A Gram-positive, facultatively anaerobic, catalase-negative, fructose-dependent strain (W13) was isolated from the gut of honeybee (). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain W13 represents a distinct line of descent within the genus , with the closest neighbours being BCRC 81240 (98.9 % sequence similarity) and DSM 19113 (96.8 % sequence similarity). Comparative sequencing of the additional phylogenetic markers and confirmed the 16S rRNA gene tree topology. The complete genome of strain W13 consisted of 1 292 712 bp with a G+C content of 48.3 mol%. Pairwise comparisons of the average nucleotide identity values and digital DNA-DNA hybridization values between the genomes of W13 and its close phylogenetic neighbours, BCRC 81240 and DSM 19113, resulted in 76.2-84.1 % and 20.2-27.6 %, respectively. The main cellular fatty acids of strain W13 were C, C 9 and C 7. Thus, we propose a novel species within the genus , with the name sp. nov. and the type strain is W13 (= NBRC 115637=BCRC 81365).
从蜜蜂肠道中分离出一株革兰氏阳性、兼性厌氧、过氧化氢酶阴性、果糖依赖型菌株（W13）。基于16S rRNA基因测序的系统发育分析表明，菌株W13代表了该属内一个独特的谱系，与其亲缘关系最近的是BCRC 81240（序列相似性为98.9%）和DSM 19113（序列相似性为96.8%）。对其他系统发育标记和的比较测序证实了16S rRNA基因树的拓扑结构。菌株W13的完整基因组由1 292 712 bp组成，G+C含量为48.3 mol%。W13与其亲缘关系密切的BCRC 81240和DSM 19113基因组之间的平均核苷酸同一性值和数字DNA-DNA杂交值的成对比较结果分别为76.2 - 84.1%和20.2 - 27.6%。菌株W13的主要细胞脂肪酸为C、C 9和C 7。因此，我们在该属内提出一个新物种，命名为sp. nov.，模式菌株为W13（=NBRC 115637=BCRC 81365）。
