College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing 100871, China.
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China.
Sci Total Environ. 2024 Jun 1;927:172110. doi: 10.1016/j.scitotenv.2024.172110. Epub 2024 Mar 31.
Recently, it is reported that bacterial communication coordinates the whole consortia to jointly resist the adverse environments. Here, we found the bacterial communication inevitably distinguished bacterial adaptation among different species in partial nitrification reactor under decreasing temperatures. We operated a partial nitrification reactor under temperature gradient from 30 °C to 5 °C and found the promotion of bacterial communication on adaptation of ammonia-oxidizing bacteria (AOB) was greater than that of nitrite-oxidizing bacteria (NOB). Signal pathways with single-component sensing protein in AOB can regulate more genes involved in bacterial adaptation than that with two-component sensing protein in NOB. The negative effects of bacterial communication, which were seriously ignored, have been highlighted, and Clp regulator downstream diffusible signal factor (DSF) based signal pathways worked as transcription activators and inhibitors of adaptation genes in AOB and NOB respectively. Bacterial communication can induce differential adaptation through influencing bacterial interactions. AOB inclined to cooperate with DSF synthesis bacteria as temperature declined, however, cooperation between NOB and DSF synthesis bacteria inclined to get weakening. According to the regulatory effects of signal pathways, bacterial survival strategies for self-protection were revealed. This study hints a potential way to govern niche differentiation in the microbiota by bacterial communication, contributing to forming an efficient artificial ecosystem.
最近有报道称，细菌通讯协调整个生物群落共同抵抗不利环境。在这里，我们发现细菌通讯不可避免地在低温下降温条件下的部分硝化反应器中区分了不同物种的细菌适应。我们在温度梯度从 30°C 到 5°C 的条件下运行部分硝化反应器，发现细菌通讯对氨氧化菌（AOB）适应的促进作用大于亚硝酸盐氧化菌（NOB）。AOB 中具有单组分感应蛋白的信号通路可以调节更多与细菌适应相关的基因，而 NOB 中具有双组分感应蛋白的信号通路则不然。我们强调了一直被严重忽视的细菌通讯的负面影响，并指出基于 Clp 调节子下游可扩散信号因子（DSF）的信号通路分别作为 AOB 和 NOB 中适应基因的转录激活剂和抑制剂发挥作用。细菌通讯可以通过影响细菌相互作用来诱导差异适应。随着温度的下降，AOB 倾向于与 DSF 合成细菌合作，然而，NOB 与 DSF 合成细菌之间的合作则倾向于减弱。根据信号通路的调节作用，揭示了细菌自我保护的生存策略。本研究提示了通过细菌通讯控制微生物群落中生态位分化的潜在途径，有助于形成高效的人工生态系统。
