Ma Fangshu, Li Anding, Li Boyin, Cui Zhibo, Shi Chunhong, Zhou Beihai
Department of Environmental Engineering, School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Environ Sci Pollut Res Int. 2015 Jul;22(14):10778-87. doi: 10.1007/s11356-015-4246-8. Epub 2015 Mar 12.
The ability of a new SBR (sequencing batch reactor) based on simulating photovoltaic aeration for maintaining nitrification activity under a 25-day starvation period was studied. The activity and abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) and the diversity of AOB were investigated. The measured biomass decay rates were 0.017 day(-1) and 0.029 day(-1) for AOB and NOB, respectively. These decay rates correlated well with AOB and NOB population quantified by real-time PCR. The recovery of ammonia oxidation rate and nitrite oxidation rate needed 4 and 7 days, respectively, indicating that NOB was more affected than AOB by starvation conditions. According to the real-time PCR results, Nitrospira was the dominant NOB in the reactor. Phylogenetic analysis indicated that Nitrosomonas oligotropha cluster was the dominant major cluster before and after starvation. Moreover, Pareto-Lorenz evenness distribution curves were plotted to interpret the interspecies abundance of AOB; the results suggested that AOB community possessed a balanced structure with medium Fo (Functional organization). Thus, the community can potentially deal with changing environmental conditions (e.g., starvation) and preserve its functionality according to the concept of functional redundancy.
研究了一种基于模拟光伏曝气的新型序批式反应器（SBR）在25天饥饿期内维持硝化活性的能力。研究了氨氧化细菌（AOB）和亚硝酸盐氧化细菌（NOB）的活性、丰度以及AOB的多样性。测得AOB和NOB的生物量衰减率分别为0.017 d⁻¹和0.029 d⁻¹。这些衰减率与通过实时PCR定量的AOB和NOB数量密切相关。氨氧化速率和亚硝酸盐氧化速率的恢复分别需要4天和7天，这表明饥饿条件对NOB的影响比对AOB的影响更大。根据实时PCR结果，硝化螺菌属是反应器中的优势NOB。系统发育分析表明，饥饿前后寡营养亚硝化单胞菌簇是主要的优势簇。此外，绘制了帕累托-洛伦兹均匀度分布曲线来解释AOB的种间丰度；结果表明，AOB群落具有中等功能组织（Fo）的平衡结构。因此，根据功能冗余的概念，该群落有可能应对不断变化的环境条件（如饥饿）并保持其功能。
