Magnusson I, Liljenberg B, Yoneyama T, Blomqvist N
J Clin Periodontol. 1985 Mar;12(3):209-15. doi: 10.1111/j.1600-051x.1985.tb00918.x.
The aim of the present investigation was to study the validity of 2 different methods of sampling the subgingival microbiota. The composition of the flora was estimated using the dark-field microscope. Subgingival samples were obtained using either a curette or a Hamilton microsyringe for a subgingival washing. The method of determination of the composition of the subgingival microorganisms was found to be very reproducible when the same investigator performed the counting. Samples obtained by the washing technique had a slight but significantly higher proportion of coccoid cells when compared to samples obtained by curette. The calculation of the reproducibility of the washing technique as assessed in 6 immediately repeated samples from the same sites yielded a coefficient of variation of 40% for spirochetes and motile rods. When 9 repeated samples from each of 28 sites (14 with a curette, 14 with washing) were studied over a period of 32 days, no systemic alterations were observed in the % of spirochetes and motile rods. The variation within each individual site was high. For curette samples and washing samples, the coefficients of variation for spirochetes and motile rods were 85% and 63%, respectively. The present investigation clearly demonstrated that care should be taken in the interpretation of single samples of subgingival microbiota. A more relevant picture of the actual situation is achieved when trends of repeated samples over time are studied.
本研究的目的是探讨两种不同的龈下微生物群采样方法的有效性。使用暗视野显微镜评估菌群的组成。使用刮匙或汉密尔顿微量注射器进行龈下冲洗以获取龈下样本。当由同一名研究人员进行计数时，发现龈下微生物组成的测定方法具有很高的可重复性。与通过刮匙获得的样本相比，通过冲洗技术获得的样本中球菌样细胞的比例略高，但具有显著差异。对来自相同部位的6个立即重复样本进行评估，冲洗技术的可重复性计算得出，螺旋体和运动杆菌的变异系数为40%。在32天的时间里，对28个部位（14个用刮匙，14个用冲洗）中的每一个部位的9个重复样本进行研究，未观察到螺旋体和运动杆菌百分比的系统性变化。每个个体部位内的变异很大。对于刮匙样本和冲洗样本，螺旋体和运动杆菌的变异系数分别为85%和63%。本研究清楚地表明，在解释龈下微生物群的单个样本时应谨慎。当研究重复样本随时间的趋势时，可以更真实地了解实际情况。
