Mullally B, Wolff L, Hardie N, Aeppli D, Pihlstrom B
Queen's University of Belfast, UK.
J Dent. 1994 Aug;22(4):223-8. doi: 10.1016/0300-5712(94)90112-0.
The purpose of this study was to determine whether gingival crevicular fluid (GCF) sampling by paper strip removes sufficient bacteria to affect subsequent subgingival plaque sampling using a curette. In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s. A greater proportion of total bacteria was removed with strip sampling at healthy rather than gingivitis or periodontitis sites. Qualitative assessment of presence or absence of spirochaetes and dark-pigmented species indicated potential for significant interference of curette sampling by the strip at gingivitis and healthy sites. We concluded that paper strip GCF sampling may significantly affect curette sampling at the same sites. The magnitude of this impact depended on the clinical classification of specific sites and the assay performed.
本研究的目的是确定用纸条采集龈沟液（GCF）是否能去除足够的细菌，从而影响随后用刮匙进行的龈下菌斑采样。在25名受试者中，分别在一个健康部位、一个牙龈炎部位和一个牙周炎部位，先用纸条采集GCF，随后用刮匙采集龈下菌斑。细菌检测表明，将纸条置于龈沟内5秒时能去除大量细菌。在健康部位进行纸条采样时，去除的细菌占总细菌的比例高于牙龈炎或牙周炎部位。对螺旋体和深色菌存在与否的定性评估表明，在牙龈炎和健康部位，纸条采样可能会对刮匙采样产生显著干扰。我们得出结论，纸条采集GCF可能会显著影响同一部位的刮匙采样。这种影响的程度取决于特定部位的临床分类以及所进行的检测。
