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对通过紫外线诱变产生的突变“巨型”体的全面理解。

Comprehensive understanding of the mutant 'giant' developed via ultraviolet mutagenesis.

作者信息

Lee Changsu, Han Sang-Il, Na Ho, Kim Zun, Ahn Joon Woo, Oh Byeolnim, Kim Hyun Soo

机构信息

Bio Division, NCell. Co., Ltd., Seoul, Republic of Korea.

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.

出版信息

Front Plant Sci. 2024 Mar 19;15:1369976. doi: 10.3389/fpls.2024.1369976. eCollection 2024.

DOI:10.3389/fpls.2024.1369976
PMID:38567133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10985164/
Abstract

INTRODUCTION

Cyanobacteria are typically of a size that can be observed under a microscope. Here, we present cyanobacteria of a size that can be observed with the naked eye. NCB002 strain showed differentiated morphological characteristics compared to previously reported spp.

METHODS

NCB002 was obtained by the UV irradiation of sp. NCB001, which was isolated from freshwater and owned by NCell Co., Ltd. NIES-39 was obtained from the National Institute for Environmental Studies (Tsukuba, Japan). We used various analytical techniques to determine its overall characteristics.

RESULTS AND DISCUSSION

The draft genome of strain NCB002 consists of five contigs comprising 6,864,973 bp with a G+C content of 44.3 mol%. The strain NCB002 had an average length of 11.69 ± 1.35 mm and a maximum of 15.15 mm, which is 23.4-50.5 times longer than the length (0.3-0.5 mm) of previously known spp., allowing it to be harvested using a thin sieve. Transcriptome analysis revealed that these morphological differences resulted from changes in cell wall formation mechanisms and increased cell division. Our results show that NCB002 has outstanding industrial value and provides a comprehensive understanding of it.

摘要

引言

蓝细菌通常具有在显微镜下可观察到的大小。在此，我们展示了肉眼可见大小的蓝细菌。与先前报道的物种相比，NCB002菌株表现出不同的形态特征。

方法

NCB002是通过对NCB001菌株进行紫外线照射获得的，NCB001从淡水中分离得到，归NCell有限公司所有。NIES - 39从日本筑波的国立环境研究所获得。我们使用了各种分析技术来确定其总体特征。

结果与讨论

NCB002菌株的基因组草图由5个重叠群组成，包含6,864,973 bp，G + C含量为44.3 mol%。NCB002菌株的平均长度为11.69 ± 1.35毫米，最长为15.15毫米，这比先前已知物种的长度（0.3 - 0.5毫米）长23.4 - 50.5倍，使其能够用细筛网收获。转录组分析表明，这些形态差异是由细胞壁形成机制的变化和细胞分裂增加导致的。我们的结果表明，NCB002具有突出的工业价值，并提供了对其的全面理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/0a213196e8c5/fpls-15-1369976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/d0316077d960/fpls-15-1369976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/d72f4c51b8c7/fpls-15-1369976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/5bf7966d390f/fpls-15-1369976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/1a4b3a6dcb9c/fpls-15-1369976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/28641c934fc1/fpls-15-1369976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/22e58bcc62d3/fpls-15-1369976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/14f5bbda1822/fpls-15-1369976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/0a213196e8c5/fpls-15-1369976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/d0316077d960/fpls-15-1369976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/d72f4c51b8c7/fpls-15-1369976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/5bf7966d390f/fpls-15-1369976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/1a4b3a6dcb9c/fpls-15-1369976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/28641c934fc1/fpls-15-1369976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/22e58bcc62d3/fpls-15-1369976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/14f5bbda1822/fpls-15-1369976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80c6/10985164/0a213196e8c5/fpls-15-1369976-g008.jpg

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