Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk University, Iksan, Republic of Korea.
Department of Animal Hospital, Hanwoo (Korean Indigenous Cattle) Genetic Improvement Center, National Agricultural Cooperative Federation, Seosan, Republic of Korea.
Vet Med Sci. 2024 May;10(3):e1434. doi: 10.1002/vms3.1434.
No tick-borne pathogens (TBPs) causing haemolytic anaemia in cattle have been reported, except Theileria orientalis and complete blood count (CBC) profile is the only haematological parameter to determine the severity of regenerative haemolytic anaemia.
To identify the causative agents of TBP-induced haemolytic anaemia and determine haematological parameters that indicate haemolytic anaemia in grazing cattle.
Eighty-two Korean indigenous cattle (Hanwoo) were divided into two groups: grazing (n = 67) and indoor (n = 15) groups. CBC and serum biochemistry were performed. PCR was conducted using whole blood-extracted DNA to investigate the prevalence of TBPs.
TBP-induced haemolytic anaemia was observed in the grazing group. In grazing cattle, co-infection (43.3%, 29/67) was most frequently detected, followed by T. orientalis (37.6%, 25/67) and Anaplasma phagocytophilum infections (1.5%, 1/67). In indoor cattle, only co-infection (20%, 3/15) was identified. Grazing cattle exhibited regenerative haemolytic anaemia with marked monocytosis, mild neutropenia, and thrombocytopenia. According to grazing frequency, the 1st-time grazing group had more severe anaemia than the 2nd-time grazing group. Elevations in indirect bilirubin and L-lactate due to haemolytic anaemia were identified, and correlations with the respective markers were determined in co-infected grazing cattle.
Quantitative evaluation of haematocrit, mean corpuscular volume, and reticulocytes (markers of regenerative haemolytic anaemia in cattle) was performed for the first time. Our results show that, in addition to T. orientalis, A. phagocytophilum is strongly associated with anaemia. The correlation between haemolytic anaemia severity and haematological parameters (indirect bilirubin, reticulocytes, and L-lactate) was confirmed.
除东方泰勒虫外，目前尚未有引起牛只溶血性贫血的蜱传病原体的报道，而全血细胞计数（CBC）是唯一能确定再生性溶血性贫血严重程度的血液学参数。
确定蜱传病原体引起的溶血性贫血的病原体，并确定指示放牧牛只发生溶血性贫血的血液学参数。
将 82 头韩国本土牛（韩牛）分为两组：放牧组（n = 67）和室内组（n = 15）。进行全血细胞计数和血清生化检查。使用从全血中提取的 DNA 进行 PCR 检查，以调查蜱传病原体的流行情况。
在放牧组中观察到蜱传病原体引起的溶血性贫血。在放牧牛中，最常检测到的是混合感染（43.3%，29/67），其次是东方泰勒虫（37.6%，25/67）和粒细胞无形体感染（1.5%，1/67）。在室内牛中，仅发现混合感染（20%，3/15）。放牧牛表现出再生性溶血性贫血，伴有明显的单核细胞增多症、轻度中性粒细胞减少症和血小板减少症。根据放牧频率，首次放牧组的贫血程度比第二次放牧组更严重。由于溶血性贫血导致间接胆红素和 L-乳酸升高，并确定了在混合感染的放牧牛中与各自标志物的相关性。
首次对牛的血液学参数（包括红细胞压积、平均红细胞体积和网织红细胞，这些都是牛再生性溶血性贫血的标志物）进行了定量评估。我们的研究结果表明，除了东方泰勒虫外，粒细胞无形体也与贫血密切相关。还证实了溶血性贫血严重程度与血液学参数（间接胆红素、网织红细胞和 L-乳酸）之间的相关性。
