Centers for Water and Health, Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai, 200032, China.
School of Public Health, Fudan University, Shanghai, 200032, China.
Anal Bioanal Chem. 2024 May;416(13):3185-3194. doi: 10.1007/s00216-024-05266-0. Epub 2024 Apr 3.
Iodoacetic acid (IAA) is a halogenated disinfection by-product of growing concern due to its high cytotoxicity, genotoxicity, endocrine disruptor effects, and potential carcinogenicity. However, the data on distribution and excretion of IAA after ingestion by mammals are still scarce. Here, we developed a reliable and validated method for detecting IAA in biological specimens (plasma, urine, feces, liver, kidney, and tissues) based on modified QuEChERS sample preparation combined with gas chromatography-tandem triple quadrupole mass spectrometry (GC-MS/MS). The detection method for IAA exhibited satisfactory recovery rates (62.6-108.0%) with low relative standard deviations (RSD < 12.3%) and a low detection limit for all biological matrices ranging from 0.007 to 0.032 ng/g. The study showed that the proposed method was reliable and reproducible for analyzing IAA in biological specimens. It was successfully used to detect IAA levels in biological samples from rats given gavage administration. The results indicated that IAA was found in various tissues and organs, including plasma, thyroid, the liver, the kidney, the spleen, gastrointestinal tract, and others, 6 h after exposure. This study provides the first data on the in vivo distribution in and excretion of IAA by mammals following oral exposure.
碘乙酸（IAA）是一种含卤素的消毒副产物，由于其高细胞毒性、遗传毒性、内分泌干扰效应和潜在致癌性，受到越来越多的关注。然而，哺乳动物摄入碘乙酸后的分布和排泄数据仍然很少。在这里，我们开发了一种基于改良 QuEChERS 样品制备结合气相色谱-串联三重四极杆质谱（GC-MS/MS）的可靠且经过验证的方法，用于检测生物样本（血浆、尿液、粪便、肝脏、肾脏和组织）中的 IAA。IAA 的检测方法具有令人满意的回收率（62.6-108.0%），相对标准偏差（RSD<12.3%）低，所有生物基质的检测限均低至 0.007-0.032ng/g。该研究表明，该方法可靠且可重现，可用于分析生物样本中的 IAA。它成功地用于检测灌胃给予碘乙酸后的大鼠生物样本中的 IAA 水平。结果表明，暴露后 6 小时，IAA 可在包括血浆、甲状腺、肝脏、肾脏、脾脏、胃肠道等在内的各种组织和器官中检测到。本研究首次提供了哺乳动物口服暴露后体内分布和排泄 IAA 的数据。
