Yang Xuemei, Yang Tiantian, Xu Yazhou
School of Chemical and Environmental Engineering, JiaoZuo University, JiaoZuo, 454000, China.
Gongyi Branch of Zhengzhou Ecological Environment Bureau, Zhengzhou, 451299, China.
Appl Biochem Biotechnol. 2024 Nov;196(11):7849-7860. doi: 10.1007/s12010-024-04923-5. Epub 2024 Apr 3.
Anaerobic fermentation of excess sludge (ES) for hydrogen production is a crucial strategy for resource utilization and environmentally friendly treatment. However, the low hydrolysis efficiency of ES and the depletion of produced hydrogen have become the limiting factors for low hydrogen yield. This study innovatively applied the bio-based surfactant alkyl polyglucoside (APG) to enhance the efficiency of dark fermentation for hydrogen production from ES. When the APG content was 100 mg/g (calculated based on total suspended solids), the maximum hydrogen production reached 17.8 mL/g VSS, approximately 3.7 times that in the control group. Mechanistic analysis revealed that APG promoted the release of organic matter from ES. APG also facilitated the release of soluble protein and soluble polysaccharide, increasing the organic matter reduction rate to 34.8%, significantly higher than other groups. APG enhanced the accumulation of volatile fatty acids and promoted the proportion of small molecular carboxylic acids. Enzyme activity analysis revealed that APG promoted the activity of hydrolytic enzymes but inhibited the activity of hydrogen-consuming enzymes. The research results provide a green and environmentally friendly strategy for the efficient resource utilization of ES.
剩余污泥厌氧发酵制氢是资源利用和环境友好型处理的关键策略。然而，剩余污泥水解效率低以及产氢量的消耗已成为产氢量低的限制因素。本研究创新性地应用生物基表面活性剂烷基糖苷（APG）来提高剩余污泥黑暗发酵产氢的效率。当APG含量为100 mg/g（基于总悬浮固体计算）时，最大产氢量达到17.8 mL/g VSS，约为对照组的3.7倍。机理分析表明，APG促进了剩余污泥中有机物的释放。APG还促进了可溶性蛋白质和可溶性多糖的释放，使有机物减少率提高到34.8%，显著高于其他组。APG增强了挥发性脂肪酸的积累并促进了小分子羧酸的比例。酶活性分析表明，APG促进了水解酶的活性，但抑制了耗氢酶的活性。研究结果为剩余污泥的高效资源利用提供了一种绿色环保策略。
