Wu Shuangshuang, Li Linman, Wang Xu, Yan Zhimin
Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.
Oral Dis. 2024 Nov;30(8):5370-5378. doi: 10.1111/odi.14934. Epub 2024 Apr 3.
Burning mouth syndrome (BMS) is a chronic orofacial pain disorder with unclear etiology, in which the tongue is most commonly affected. This study aims to provide implication of the possible relationship between oral microbiota and the pathogenesis of BMS.
Saliva and tongue swabs of 15 primary BMS patients and 10 healthy controls were collected and assessed by 16S rRNA gene amplicon sequencing. The microbiota compositions were compared and bioinformatic analysis was conducted.
Differences in microbiota compositions between BMS patients and healthy controls were revealed in both saliva and tongue samples. In saliva, Streptococcus, Rothia, and Neisseria were the predominant genus at the taxonomic level in BMS patients. In tongue samples, Prevotella, Streptococcus, and Neisseria were the dominant genus at the taxonomic level in BMS patients. LEfSe analysis and linear discriminant analysis score showed that Actinobacteria were the predominant phylum in saliva, and Selenomonas were enriched in the dorsum of the tongue of BMS patients.
This study for the first-time reported saliva and tongue microbiota profiles were distinguished from that of healthy controls, indicating a necessity for further research on the possible relationship between oral microbes and the pathogenesis of BMS.
灼口综合征（BMS）是一种病因不明的慢性口腔面部疼痛疾病，其中舌头最常受累。本研究旨在探讨口腔微生物群与BMS发病机制之间可能存在的关系。
收集15例原发性BMS患者和10例健康对照者的唾液和舌拭子，采用16S rRNA基因扩增子测序进行评估。比较微生物群组成并进行生物信息学分析。
在唾液和舌样本中均发现BMS患者与健康对照者的微生物群组成存在差异。在唾液中，链球菌属、罗氏菌属和奈瑟菌属是BMS患者分类水平上的主要菌属。在舌样本中，普雷沃菌属、链球菌属和奈瑟菌属是BMS患者分类水平上的优势菌属。LEfSe分析和线性判别分析评分显示，放线菌门是唾液中的主要菌门，而在BMS患者的舌背中，栖瘤胃普雷沃菌属富集。
本研究首次报道唾液和舌微生物群谱与健康对照者不同，表明有必要进一步研究口腔微生物与BMS发病机制之间的可能关系。
