The Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Joint Laboratory of Drug Delivery & Innovative Therapy Built by Beijing Chaoyang Hospital & State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
Retina. 2024 Aug 1;44(8):1449-1455. doi: 10.1097/IAE.0000000000004119.
To evaluate the utility of galactomannan testing of intraocular fluid in the diagnosis of Aspergillus endophthalmitis (AE).
This retrospective study enrolled three groups of patients, including those with 17 eyes with AE; 20 eyes with intraocular infection of bacteria, viruses, or other fungi; and 19 eyes with cataract. Intraocular fluid from all these patients was collected for galactomannan testing. In addition, the receiver operating characteristic curves and diagnostic significance were analyzed.
The mean optical density index (ODI) of galactomannan was 5.77 ± 1.73 in the AE group, which was significantly higher than that in the non- Aspergillus intraocular infection group (0.19 ± 0.11, P < 0.001) and the negative control group (0.29 ± 0.27, P < 0.001). The area under the receiver operating characteristic curve (area under the curve) was 1.00 (95% confidence interval, 1.00-1.00; P < 0.001) in the AE group and the other two groups. At a cutoff optical density index of 1.88, the sensitivity and specificity were 100.0% and 100.0%, respectively, and the Youden index reached its highest value of 1.00.
Galactomannan testing of intraocular fluid indicated good sensitivity and specificity for the diagnosis of AE, thereby promising a rapid diagnostic modality for AE.
评估眼内液半乳甘露聚糖检测在诊断曲霉菌眼内炎（AE）中的应用价值。
本回顾性研究纳入了三组患者，包括 17 只眼的 AE 患者、20 只眼的眼部细菌、病毒或其他真菌感染患者和 19 只眼的白内障患者。所有患者的眼内液均用于进行半乳甘露聚糖检测。此外，还分析了受试者工作特征曲线和诊断意义。
AE 组的半乳甘露聚糖平均光密度指数（ODI）为 5.77 ± 1.73，明显高于非曲霉菌眼内感染组（0.19 ± 0.11，P < 0.001）和阴性对照组（0.29 ± 0.27，P < 0.001）。AE 组和其他两组的受试者工作特征曲线下面积（AUC）均为 1.00（95%置信区间，1.00-1.00；P < 0.001）。当截断光密度指数为 1.88 时，敏感性和特异性分别为 100.0%和 100.0%，Youden 指数达到最高值 1.00。
眼内液半乳甘露聚糖检测对诊断 AE 具有良好的敏感性和特异性，有望成为一种快速诊断 AE 的方法。
