College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Talanta. 2024 Jul 1;274:125997. doi: 10.1016/j.talanta.2024.125997. Epub 2024 Apr 2.
Cyanidin-3-O-glucoside (C3G), a natural antioxidant, plays multiple physiological or pathological roles in maintaining human health; thereby, designing advanced sensors to achieve specific recognition and high-sensitivity detection of C3G is significant. Herein, an imprinted-type electrochemiluminescence (ECL) sensing platform was developed using core-shell Ru@SiO-CMIPs, which were prepared by covalent organic framework (COF)-based molecularly imprinted polymers (CMIPs) embedded in luminescent Ru@SiO cores. The C3G-imprinted COF shell not only helps generate a steady-enhanced ECL signal, but also enables specific recognition of C3G. When C3G is bound to Ru@SiO-CMIPs with abundant imprinted cavities, resonance energy transfer (RET) behavior is triggered, resulting in a quenched ECL response. The constructed Ru@SiO-CMIPs nanoprobes exhibit ultra-high sensitivity, absolute specificity, and an ultra-low detection limit (0.15 pg mL) for analyzing C3G in food matrices. This study provides a means to construct an efficient and reliable molecular imprinting-based ECL sensor for food analysis.
矢车菊素-3-O-葡萄糖苷（C3G）是一种天然抗氧化剂，在维持人体健康方面发挥着多种生理或病理作用；因此，设计先进的传感器来实现 C3G 的特异性识别和高灵敏度检测具有重要意义。在此，通过将基于共价有机骨架（COF）的分子印迹聚合物（CMIPs）嵌入发光 Ru@SiO 核中，制备了核壳型 Ru@SiO-CMIPs，并用其开发了印迹型电化学发光（ECL）传感平台。C3G 印迹的 COF 壳不仅有助于产生稳定增强的 ECL 信号，而且还能够对 C3G 进行特异性识别。当 C3G 与具有丰富印迹腔的 Ru@SiO-CMIPs 结合时，会触发共振能量转移（RET）行为，导致 ECL 响应被猝灭。构建的 Ru@SiO-CMIPs 纳米探针在分析食品基质中的 C3G 时表现出超高的灵敏度、绝对的特异性和超低的检测限（0.15 pg mL）。本研究为构建用于食品分析的高效、可靠的基于分子印迹的 ECL 传感器提供了一种方法。
