Wayne State University, Department of Biological Sciences, Detroit, MI, USA; Wayne State University, Department of Chemistry, Detroit, MI, USA.
Wayne State University, Department of Biological Sciences, Detroit, MI, USA.
Curr Opin Microbiol. 2024 Jun;79:102467. doi: 10.1016/j.mib.2024.102467. Epub 2024 Apr 3.
Bacterial cells have a unique challenge to organize their cytoplasm without the use of membrane-bound organelles. Biomolecular condensates (henceforth BMCs) are a class of nonmembrane-bound organelles, which, through the physical process of phase separation, can form liquid-like droplets with proteins/nucleic acids. BMCs have been broadly characterized in eukaryotic cells, and BMCs have been recently identified in bacteria, with the first and best studied example being bacterial ribonucleoprotein bodies (BR-bodies). BR-bodies contain the RNA decay machinery and show functional parallels to eukaryotic P-bodies (PBs) and stress granules (SGs). Due to the finding that mRNA decay machinery is compartmentalized in BR-bodies and in eukaryotic PBs/SGs, we will explore the functional similarities in the proteins, which are known to enrich in these structures based on recent proteomic studies. Interestingly, despite the use of different mRNA decay and post-transcriptional regulatory machinery, this analysis has revealed evolutionary convergence in the classes of enriched enzymes in these structures.
细菌细胞在没有膜结合细胞器的情况下组织细胞质具有独特的挑战。生物分子凝聚物（以下简称 BMCs）是一类非膜结合细胞器，通过相分离的物理过程，可形成具有蛋白质/核酸的液态滴。BMCs 在真核细胞中得到了广泛的描述，并且最近在细菌中也发现了 BMCs，第一个也是研究得最好的例子是细菌核糖核蛋白体（BR-bodies）。BR-bodies 包含 RNA 降解机制，并与真核 P 体（PBs）和应激颗粒（SGs）表现出功能上的相似性。由于发现 mRNA 降解机制在 BR-bodies 和真核 PBs/SGs 中被分隔开，我们将根据最近的蛋白质组学研究探索这些结构中已知富含的蛋白质的功能相似性。有趣的是，尽管使用了不同的 mRNA 降解和转录后调控机制，但这项分析揭示了这些结构中富集酶类的进化趋同。
