College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Macromol Biosci. 2024 Jul;24(7):e2400071. doi: 10.1002/mabi.202400071. Epub 2024 Apr 10.
Engineered nanomaterials are promising in biomedical application. However, insufficient understanding of their biocompatibility at the cellular and organic levels prevents their widely biomedical applications. Metal-organic frameworks (MOFs) have attracted increasing attention in recent years. In this work, zeolitic imidazolate framework-8 (ZIF-8) and polydopamine (PDA)-modified ZIF-8 are chosen as model nanomaterials due to its emergent role in nanomedicine. In vitro, the results demonstrate that the PDA coating greatly alleviates the cytotoxicity of ZIF-8 to RAW264.7, LO2, and HST6, which represent three different cell types in liver organs. Mechanistically, ZIF-8 entering into the cells can greatly induce the reactive oxygen species generation, which subsequently induces cell cycle delay and autophagy, ultimately leads to enhanced cytotoxicity. Further, human umbilical vein endothelial cells model and zebrafish embryos assay also confirm that PDA can compromise the ZIF-8 toxicity significantly. This study reveals that PDA-coated MOFs nanomaterials show great potential in nano-based drug delivery systems .
工程纳米材料在生物医学应用中具有广阔的前景。然而，由于人们对其在细胞和有机水平上的生物相容性缺乏充分的了解，限制了其在生物医学领域的广泛应用。金属有机框架（MOFs）近年来受到了越来越多的关注。在这项工作中，沸石咪唑酯骨架-8（ZIF-8）和聚多巴胺（PDA）修饰的 ZIF-8被选择为模型纳米材料，因为它们在纳米医学中具有重要作用。体外实验结果表明，PDA 涂层能显著减轻 ZIF-8 对 RAW264.7、LO2 和 HST6 的细胞毒性，这三种细胞分别代表了肝脏中的三种不同细胞类型。从机制上讲，进入细胞的 ZIF-8 能极大地诱导活性氧的产生，进而导致细胞周期延迟和自噬，最终导致细胞毒性增强。此外，人脐静脉内皮细胞模型和斑马鱼胚胎试验也证实，PDA 能显著减轻 ZIF-8 的毒性。本研究揭示了 PDA 涂层的 MOFs 纳米材料在基于纳米的药物输送系统中有很大的应用潜力。
