日本千叶县牛乳中乳链菌分离株的抗菌药物耐药模式：多药耐药性与 996 克隆复合体之间的关联。
Antimicrobial resistance patterns of Streptococcus uberis isolates from bovine milk in Chiba prefecture, Japan: association between multidrug resistance and clonal complex 996.
机构信息
Division of Clinical Laboratory, Sanritsu Zelkova Veterinary Laboratory, Tokyo, Japan.
Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo, Japan.
出版信息
J Vet Med Sci. 2024 May 6;86(5):468-473. doi: 10.1292/jvms.23-0526. Epub 2024 Apr 2.
Streptococcus uberis is one of major pathogens causing bovine mastitis. However, there is poor information on antimicrobial resistance (AMR) among the Japanese isolates. To provide treatment information for the mastitis caused by S. uberis in Japan, we aimed to clarify AMR patterns of the isolates from bovine milk mainly in Chiba. AMR phenotyping/genotyping [blaZ-erm(A)-erm(B)-mef(A)-linB-lnuD-tet(M)-tet(O)-tet(K)-tet(L)-tet(S)] and multilocus sequence typing were performed to analyze relationships between AMR patterns and clonal complexes (CCs). Resistance to tetracycline-, macrolide-, and lincosamide-classes was mainly associated with possession of tet(O), tet(S), erm(B), linB, and lnuD genes. CC996 was significantly associated with multidrug resistance (P<0.0001). These findings will aid Chiba farm animal clinics in treating bovine mastitis.
乳房链球菌是引起奶牛乳腺炎的主要病原体之一。然而，关于日本分离株的抗菌药物耐药性（AMR）的信息很少。为了为日本乳房链球菌引起的乳腺炎提供治疗信息，我们旨在阐明主要来自千叶的牛乳中分离株的 AMR 模式。进行了 AMR 表型/基因型 [blaZ-erm(A)-erm(B)-mef(A)-linB-lnuD-tet(M)-tet(O)-tet(K)-tet(L)-tet(S)] 和多位点序列分型，以分析 AMR 模式与克隆复合体（CC）之间的关系。对四环素类、大环内酯类和林可酰胺类的耐药性主要与 tet(O)、tet(S)、erm(B)、linB 和 lnuD 基因的存在有关。CC996 与多药耐药性显著相关（P<0.0001）。这些发现将有助于千叶农场动物诊所治疗奶牛乳腺炎。
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