Cho M I, Garant P R
Arch Oral Biol. 1985;30(2):111-20. doi: 10.1016/0003-9969(85)90102-5.
[3H]-fucose utilization by odontoblasts was studied by light and electron microscopic radioautography. At 10 min after injection, fucose label was concentrated in the Golgi area. By 20-30 min, there was a progressive decline in Golgi labelling with label present at the plasma membrane, terminal web, odontoblast process and predentine matrix. At 4 h, the predentine and the predentine-dentine junction were heavily labelled. At the ultrastructural level, Golgi labelling at 10 min was mostly localized to cisternal elements and at 20 and 30 min secretory granules and dense bodies were also labelled. Most of the silver grains observed in the terminal web were associated with microfilaments near the plasma membrane. In the predentine, the matrix itself accounted for 23.0 per cent of the label at 4 h and the plasma membrane of the odontoblast process accounted for 19 per cent. The results indicate that odontoblasts, in addition to secreting glycoproteins into the dentinal matrix, also continuously manufacture glycoproteins for incorporation into the cell surface, the lysosomal system and the terminal web.
通过光学显微镜和电子显微镜放射自显影术研究了成牙本质细胞对[3H]-岩藻糖的利用情况。注射后10分钟，岩藻糖标记物集中在高尔基体区域。到20 - 30分钟时，高尔基体标记逐渐减少，标记物出现在质膜、终末网、成牙本质细胞突起和前期牙本质基质中。4小时时，前期牙本质和前期牙本质 - 牙本质交界处被大量标记。在超微结构水平上，10分钟时高尔基体标记主要定位于扁平囊泡成分，20和30分钟时分泌颗粒和致密体也被标记。在终末网中观察到的大多数银颗粒与质膜附近的微丝相关。在前期牙本质中，4小时时基质本身占标记物的23.0%，成牙本质细胞突起的质膜占19%。结果表明，成牙本质细胞除了向牙本质基质分泌糖蛋白外，还持续合成糖蛋白以整合到细胞表面、溶酶体系统和终末网中。
