NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Methods Mol Biol. 2024;2797:145-157. doi: 10.1007/978-1-0716-3822-4_11.
MALDI-TOF mass spectrometry enables high-throughput screening of covalent fragment libraries and SAR compound progressions of selective KRAS G12C inhibitors. Using the MALDI-TOF platform instead of the more traditional ESI-MS TOF/orbitrap instrumentation can radically shorten sample acquisition time, allowing up to 384 samples to be screened in 30 min. The typical throughput for a covalent library screen is 1152 samples per 8 h, including processing, calculation, and reporting steps. The throughput can be doubled without any significant assay modification.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）能够高通量筛选共价片段文库和选择性 KRAS G12C 抑制剂的 SAR 化合物进展。与更传统的 ESI-MS TOF/Orbitrap 仪器相比，使用 MALDI-TOF 平台可以极大地缩短样品采集时间，允许在 30 分钟内筛选多达 384 个样品。共价文库筛选的典型通量为每 8 小时 1152 个样品，包括处理、计算和报告步骤。在不进行重大测定修改的情况下，通量可以翻倍。
