Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, 1-8-14, Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8308, Japan.
NAC Techno-Science Co., Ltd, Tokyo, 165-0024, Japan.
Anal Sci. 2024 Jul;40(7):1365-1371. doi: 10.1007/s44211-024-00552-4. Epub 2024 Apr 4.
Suppressed ion chromatography with perchloric acid deproteinization was developed for the determination of phosphorus in commercially available milk. Although the perchloric acid deproteinization method is widely used in the medical field, it sees limited application in the food industry. Herein, the concentration of perchloric acid and hydrolysis conditions were examined, specifically regarding perchloric acid deproteinization, which was used as a deproteinization method in this study. The calibration curve constructed from the peak area of orthophosphoric acid (monohydrogen phosphate ion: HPO) was linear, with a correlation coefficient of 0.999. The relative standard deviation of the peak area of 50 mg/L of HPO from six replicates was 0.35%. The detection and quantitative limits of HPO, calculated from its signal-to-noise ratio were 0.033 mg/L and 0.100 mg/L, respectively. The proposed method was applied to the analysis of phosphorus in commercially available milk. Perchloric acid deproteinization has proved to be useful in the food industry.
采用高氯酸消解法抑制离子色谱法测定市售牛奶中的磷。虽然高氯酸消解法在医学领域被广泛应用，但在食品工业中应用有限。本研究中，考察了高氯酸的浓度和水解条件，特别是高氯酸消解法作为一种蛋白质沉淀方法。由正磷酸（单氢磷酸离子：HPO）峰面积构建的校准曲线呈线性，相关系数为 0.999。从六个重复中 HPO 峰面积的相对标准偏差为 0.35%。根据信号噪声比计算出 HPO 的检测限和定量限分别为 0.033 mg/L 和 0.100 mg/L。该方法应用于市售牛奶中磷的分析。高氯酸消解法已被证明在食品工业中是有用的。
