采用抑制型离子色谱法测定葡萄酒中的亚硫酸盐。

Determination of sulphite in wines using suppressed ion chromatography.

作者信息

Yoshikawa Kenji, Uekusa Yuki, Sakuragawa Akio

机构信息

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, 1-5-1, Kanda-Surugadai, Chiyoda, Tokyo 101-8308, Japan.

出版信息

Food Chem. 2015 May 1;174:387-91. doi: 10.1016/j.foodchem.2014.11.066. Epub 2014 Nov 15.

DOI:10.1016/j.foodchem.2014.11.066

PMID:25529696

Abstract

Suppressed ion chromatography with the use of a conductivity detector was developed for the determination of sulphite ions in wine samples. When a mixed solution of sodium carbonate, sodium bicarbonate, and acetone was used as the mobile phase, simultaneous determination of eight inorganic anions (i.e., fluoride, chloride, nitrite, nitrate, sulphite, phosphate, sulphate, and thiosulphate) was completed in approximately 25 min. Linearity, reproducibility, and detection limits were determined for the proposed method. In the case of sulphite detection, a linear calibration curve with a good correlation coefficient of 0.9992 was obtained from the peak height of sulphite with a relative standard deviation (n = 6) 1.48%. In addition, the detection limit of sulphite was 0.27 mg/L at a signal-to-noise ratio of 3. Further, the developed method was applied for the determination of sulphite contained in several wine samples.

摘要

采用抑制型离子色谱结合电导检测器，开发了一种用于测定葡萄酒样品中亚硫酸根离子的方法。当使用碳酸钠、碳酸氢钠和丙酮的混合溶液作为流动相时，可在约25分钟内同时测定8种无机阴离子（即氟离子、氯离子、亚硝酸根、硝酸根、亚硫酸根、磷酸根、硫酸根和硫代硫酸根）。对所提出的方法进行了线性、重现性和检测限的测定。在亚硫酸根检测方面，根据亚硫酸根的峰高获得了相关系数为0.9992的线性校准曲线，相对标准偏差（n = 6）为1.48%。此外，在信噪比为3时，亚硫酸根的检测限为0.27 mg/L。此外，所开发的方法还应用于测定几种葡萄酒样品中所含的亚硫酸根。