Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment. the Hebrew University of Jerusalem, Israel; Department of Natural and Life Sciences, The Open University of Israel, Israel.
Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment. the Hebrew University of Jerusalem, Israel.
Fungal Biol. 2024 Apr;128(2):1714-1723. doi: 10.1016/j.funbio.2024.02.007. Epub 2024 Mar 1.
The repair capacity of ultra-violet (UV) light DNA damage is important for adaptation of fungi to different ecological niches. We previously showed that in the soil-borne pathogen Fusarium oxysporum photo-reactivation dependent UV repair is induced at the germling stage and reduced at the filament stage. Here, we tested the developmental control of the transcription of photolyase, UV survival, UV repair capacity, and UV induced mutagenesis in the foliar pathogen Fusarium mangiferae. Unlike F. oxysporum, neither did we observe developmental control over photo-reactivation dependent repair nor the changes in gene expression of photolyase throughout the experiment. Similarly, photo-reactivation assisted reduction in UV induced mutagenesis was similar throughout the development of F. mangiferae but fluctuated during the development of F. oxysporum. To generate hypotheses regarding the recovery of F. mangiferae after UV exposure, an RNAseq analysis was performed after irradiation at different timepoints. The most striking effect of UV on F. mangiferae was developmental-dependent induction of translation related genes. We further report a complex response that changes during recovery time and involves translation, cell cycle and lipid biology related genes.
紫外线 (UV) 光损伤的修复能力对真菌适应不同生态位非常重要。我们之前曾表明，在土壤病原体尖孢镰刀菌中，光复活依赖的 UV 修复在萌芽阶段被诱导，而在丝状阶段则减少。在这里，我们测试了叶病原菌芒果炭疽菌中光解酶转录、UV 存活、UV 修复能力和 UV 诱导突变的发育控制。与尖孢镰刀菌不同，我们在整个实验过程中既没有观察到光复活依赖的修复的发育控制，也没有观察到光解酶基因表达的变化。同样，光复活辅助降低 UV 诱导的突变在芒果炭疽菌的整个发育过程中相似，但在尖孢镰刀菌的发育过程中波动。为了针对 UV 暴露后芒果炭疽菌的恢复生成假设，我们在不同时间点照射后进行了 RNAseq 分析。UV 对芒果炭疽菌最显著的影响是与翻译相关的基因的发育依赖性诱导。我们进一步报告了一个在恢复时间过程中发生变化的复杂反应，涉及翻译、细胞周期和脂质生物学相关基因。
