Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.
Shaanxi Provincial Key Laboratory of Magnetic Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Sci Rep. 2024 Apr 4;14(1):7907. doi: 10.1038/s41598-024-55466-y.
Vesicovaginal fistula lacks a standard, established animal model, making surgical innovations for this condition challenging. Herein, we aimed to non-surgically establish vesicovaginal fistula using the magnetic compression technique, and the feasibility of this method was explored using eight female Beagle dogs as model animals. In these dogs, cylindrical daughter and parent magnets were implanted into the bladder and vagina, respectively, after anesthesia, and the positions of these magnets were adjusted under X-ray supervision to make them attract each other, thus forming the structure of daughter magnet-bladder wall-vaginal wall-parent magnet. Operation time and collateral damage were recorded. The experimental animals were euthanized 2 weeks postoperatively, and the vesicovaginal fistula gross specimens were obtained. The size of the fistula was measured. Vesicovaginal fistula was observed by naked eye and under a light microscope. Magnet placement was successful in all dogs, and remained in the established position for the reminder of the experiment. The average operation time was 14.38 min ± 1.66 min (range, 12-17 min). The dogs were generally in good condition postoperatively and were voiding normally, with no complications like bleeding and urine retention. The magnets were removed from the vagina after euthanasia. The vesicovaginal fistula was successfully established according to gross observation, and the fistula diameters were 4.50-6.24 mm. Histological observation revealed that the bladder mucosa and vaginal mucosa were in close contact on the internal surface of the fistula. Taken together, magnetic compression technique is a simple and feasible method to establish an animal model of vesicovaginal fistula using Beagle dogs. This model can help clinicians study new surgical techniques and practice innovative approaches for treating vesicovaginal fistula.
膀胱阴道瘘缺乏标准的、已建立的动物模型，这使得该疾病的手术创新具有挑战性。在此，我们旨在使用磁压缩技术非手术建立膀胱阴道瘘，并使用 8 只雌性比格犬作为模型动物探索这种方法的可行性。在这些犬中，在麻醉后分别将圆柱形子磁体和母磁体植入膀胱和阴道中，并在 X 射线监视下调整这些磁体的位置，使其相互吸引，从而形成子磁体-膀胱壁-阴道壁-母磁体的结构。记录手术时间和副损伤。术后 2 周处死实验动物，获取膀胱阴道瘘大体标本。测量瘘口大小。肉眼和光镜下观察膀胱阴道瘘。所有犬的磁体放置均成功，且在实验过程中一直保持在建立的位置。平均手术时间为 14.38 分钟±1.66 分钟（范围为 12-17 分钟）。术后犬一般状况良好，排尿正常，无出血、尿潴留等并发症。安乐死后从阴道中取出磁铁。根据大体观察成功建立了膀胱阴道瘘，瘘口直径为 4.50-6.24 毫米。组织学观察显示，瘘管内表面膀胱黏膜和阴道黏膜紧密接触。综上所述，磁压缩技术是使用比格犬建立膀胱阴道瘘动物模型的一种简单可行的方法。该模型有助于临床医生研究新的手术技术，并为治疗膀胱阴道瘘实践创新方法。
