Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, No.150 of Qingta, Fengtai District, Beijing, 100141, People's Republic of China.
Environ Sci Pollut Res Int. 2024 Apr;31(20):29525-29535. doi: 10.1007/s11356-024-33142-y. Epub 2024 Apr 5.
Antibiotics have been widely detected in aquatic environments, and fungal biotransformation receives considerable attention for antibiotic bioremediation. Here, a fungus designated Cladosporium cladosporioides 11 (CC11) with effective capacity to biotransform fluoroquinolones was isolated from aquaculture pond sediments. Enrofloxacin (ENR), ciprofloxacin (CIP) and ofloxacin (OFL) were considerably abated by CC11, and the antibacterial activities of the fluoroquinolones reduced significantly after CC11 treatment. Transcriptome analysis showed the removal of ENR, CIP and OFL by CC11 is a process of enzymatic degradation and biosorption which consists well with ligninolytic enzyme activities and sorption experiments under the same conditions. Additionally, CC11 significantly removed ENR in zebrafish culture water and reduced the residue of ENR in zebrafish. All these results evidenced the potential of CC11 as a novel environmentally friendly process for the removal of fluoroquinolones from aqueous systems and reduce fluoroquinolone residues in aquatic organisms.
抗生素已广泛存在于水生环境中，真菌生物转化因其在抗生素生物修复方面的潜力而受到广泛关注。本研究从水产养殖池塘底泥中分离到一株可有效转化氟喹诺酮类抗生素的真菌，命名为 Cladosporium cladosporioides 11（CC11）。CC11 对恩诺沙星（ENR）、环丙沙星（CIP）和氧氟沙星（OFL）具有较强的转化能力，经 CC11 处理后，氟喹诺酮类抗生素的抗菌活性显著降低。转录组分析表明，CC11 对 ENR、CIP 和 OFL 的去除是一个酶降解和生物吸附的过程，这与相同条件下木质素过氧化物酶活性和吸附实验结果一致。此外，CC11 可有效去除斑马鱼养殖水中的 ENR，并降低 ENR 在斑马鱼体内的残留。这些结果均表明 CC11 可作为一种新型的环保工艺，用于从水体中去除氟喹诺酮类抗生素，并降低水生生物中氟喹诺酮类抗生素的残留。
