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Effect of regional DNA methylation on gene expression.

作者信息

Keshet I, Yisraeli J, Cedar H

出版信息

Proc Natl Acad Sci U S A. 1985 May;82(9):2560-4. doi: 10.1073/pnas.82.9.2560.

DOI:10.1073/pnas.82.9.2560
PMID:3857599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397603/
Abstract

The effect of DNA methylation on the transcriptional activity of the hamster adenine phosphoribosyltransferase (aprt) and the herpes thymidine kinase (tk) genes has been investigated. By using M13 constructs containing these gene sequences, specific segments of each gene were methylated in vitro by restriction fragment primer-directed second-strand synthesis using the substrate 2'-deoxy-5-methyl-cytidine triphosphate (dmCTP). These hybrid-methylated molecules were inserted into mouse Ltk- cells by DNA-mediated cotransfer. In all cases, the integrated sequences retained the in vitro-directed methylation pattern. The aprt gene was inhibited by CpG methylation in the 5' region but was unaffected by methylation at the 3' end or in adjacent M13 sequences. In contrast to this, DNA methylation in both the 5' promoter region and the 3' structural region of the tk gene had a strong inhibitory effect. This suggests that this modification may affect transcription by mechanisms that do not involve the direct alteration of recognition sequences for RNA polymerase.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/222a6092cd50/pnas00349-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/56976e921735/pnas00349-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/e3da84bdf375/pnas00349-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/5097e9ec2a4a/pnas00349-0016-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/c0d11b474b9c/pnas00349-0016-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/2e12cd8bc82f/pnas00349-0016-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/4a8231c31e42/pnas00349-0016-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/874abb4357cc/pnas00349-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/222a6092cd50/pnas00349-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/56976e921735/pnas00349-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/e3da84bdf375/pnas00349-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/5097e9ec2a4a/pnas00349-0016-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/c0d11b474b9c/pnas00349-0016-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/2e12cd8bc82f/pnas00349-0016-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/4a8231c31e42/pnas00349-0016-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/874abb4357cc/pnas00349-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d656/397603/222a6092cd50/pnas00349-0017-b.jpg

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本文引用的文献

1
Isolation of transforming DNA: cloning the hamster aprt gene.转化DNA的分离：仓鼠aprt基因的克隆
Cell. 1980 Dec;22(3):817-23. doi: 10.1016/0092-8674(80)90558-9.
2
Undermethylation at the 5' end of the albumin gene is necessary but not sufficient for albumin production by rat hepatoma cells in culture.白蛋白基因5'端的低甲基化对于培养的大鼠肝癌细胞产生白蛋白是必要的，但并不充分。
Cell. 1982 Oct;30(3):825-33. doi: 10.1016/0092-8674(82)90287-2.
3
Estrogen induces a demethylation at the 5' end region of the chicken vitellogenin gene.雌激素可诱导鸡卵黄蛋白原基因5'端区域发生去甲基化。
Nat Commun. 2024 Aug 6;15(1):6690. doi: 10.1038/s41467-024-50850-8.
4
The role of noncoding RNAs in pancreatic birth defects.非编码 RNA 在胰腺发育缺陷中的作用。
Birth Defects Res. 2023 Nov 15;115(19):1785-1808. doi: 10.1002/bdr2.2178. Epub 2023 Apr 17.
5
The sound of silence: Transgene silencing in mammalian cell engineering.沉默的声音：哺乳动物细胞工程中的转基因沉默。
Cell Syst. 2022 Dec 21;13(12):950-973. doi: 10.1016/j.cels.2022.11.005.
6
Allele-specific cis-regulatory methylation of the gene for vasoactive intestinal peptide in white-throated sparrows.血管活性肠肽基因的等位基因特异性顺式调控甲基化在白喉雀中。
Genes Brain Behav. 2022 Nov;21(8):e12831. doi: 10.1111/gbb.12831. Epub 2022 Oct 11.
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Neurogenesis in the neonatal rat hippocampus is regulated by sexually dimorphic epigenetic modifiers.新生大鼠海马中的神经发生受性别二态性表观遗传修饰物的调控。
Biol Sex Differ. 2022 Mar 7;13(1):9. doi: 10.1186/s13293-022-00418-2.
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Distinct DNA Methylation Patterns of Subependymal Giant Cell Astrocytomas in Tuberous Sclerosis Complex.结节性硬化症患者室管膜下巨细胞星形细胞瘤的独特 DNA 甲基化模式。
Cell Mol Neurobiol. 2022 Nov;42(8):2863-2892. doi: 10.1007/s10571-021-01157-5. Epub 2021 Oct 28.
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and Gene Expression Changes in Deoxynivalenol Treated IPEC-J2 Cells.脱氧雪腐镰刀菌烯醇处理的IPEC-J2细胞中的基因表达变化
Front Genet. 2021 Jul 21;12:697883. doi: 10.3389/fgene.2021.697883. eCollection 2021.
10
DNA methylation as the link between migration and the major noncommunicable diseases: the RODAM study.DNA 甲基化作为迁移与主要非传染性疾病之间的联系：RODAM 研究。
Epigenomics. 2021 May;13(9):653-666. doi: 10.2217/epi-2020-0329. Epub 2021 Apr 23.
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4252-5. doi: 10.1073/pnas.79.14.4252.
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In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.仓鼠腺嘌呤磷酸核糖转移酶基因的体外甲基化抑制其在小鼠L细胞中的表达。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3418-22. doi: 10.1073/pnas.79.11.3418.
5
Expression of a cloned adenovirus gene is inhibited by in vitro methylation.克隆腺病毒基因的表达受到体外甲基化的抑制。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1073-7. doi: 10.1073/pnas.79.4.1073.
6
DNA methylation and the regulation of globin gene expression.DNA甲基化与珠蛋白基因表达的调控
Cell. 1983 Aug;34(1):197-206. doi: 10.1016/0092-8674(83)90150-2.
7
Primary activation of cytosolic phosphoenolpyruvate carboxykinase gene in fetal rat liver and the biogenesis of its mRNA.胎鼠肝脏中胞质磷酸烯醇式丙酮酸羧激酶基因的初级激活及其mRNA的生物合成。
Eur J Biochem. 1984 May 15;141(1):199-203. doi: 10.1111/j.1432-1033.1984.tb08175.x.
8
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Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:605-9. doi: 10.1101/sqb.1983.047.01.071.
9
Clonal inheritance of the pattern of DNA methylation in mouse cells.小鼠细胞中DNA甲基化模式的克隆遗传。
Proc Natl Acad Sci U S A. 1982 Jan;79(1):61-5. doi: 10.1073/pnas.79.1.61.
10
Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression.氯霉素乙酰转移酶基因在12型腺病毒启动子控制下在哺乳动物细胞中的表达：启动子甲基化对基因表达的影响。
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7586-90. doi: 10.1073/pnas.80.24.7586.