Mencher D, Cohen H, Benvenisty N, Meyuhas O, Reshef L
Eur J Biochem. 1984 May 15;141(1):199-203. doi: 10.1111/j.1432-1033.1984.tb08175.x.
The primary appearance of phosphoenolpyruvate (P-pyruvate) carboxykinase RNA transcripts in fetal liver was induced by a number of different stimulii . This may occur as rapidly as an hour after injection in utero of N6,O2'-dibutyryl-adenosine 3',5'-monophosphate (Bt2cAMP) to fetuses, suggesting that all stimulii predominantly affect activation of the P-pyruvate carboxykinase gene. Bt2cAMP treatment induces the appearance of the enzyme RNA transcripts, predominantly of the mature type in the cytoplasm. However, insulin deficiency by streptozotocin treatment causes the appearance of large-size as well as mature mRNA in the nucleus, in addition to the appearance of P-pyruvate carboxykinase mRNA in the cytoplasm. Insulin treatment of such diabetic fetuses, prior to causing the disappearance of P-pyruvate carboxykinase mRNA, reduces nuclear transcripts but increases the abundance of mature cytoplasmic enzyme mRNA. Bt2cAMP treatment of insulin-deficient fetuses causes an additive effect, increasing the abundance of not only the mature but the large P-pyruvate carboxykinase RNA transcripts as well. The results are best interpreted as insulin acting both to inhibit transcription of and accelerate post-transcriptional processes affecting P-pyruvate carboxykinase RNA.
磷酸烯醇丙酮酸（P-丙酮酸）羧激酶RNA转录本在胎儿肝脏中的最初出现是由多种不同刺激诱导的。在子宫内给胎儿注射N6,O2'-二丁酰腺苷3',5'-单磷酸（Bt2cAMP）后，可能在一小时内就会迅速出现这种情况，这表明所有刺激主要影响P-丙酮酸羧激酶基因的激活。Bt2cAMP处理会诱导该酶RNA转录本的出现，主要是细胞质中的成熟型。然而，链脲佐菌素处理导致的胰岛素缺乏除了使细胞质中出现P-丙酮酸羧激酶mRNA外，还会使细胞核中出现大尺寸以及成熟的mRNA。对这类糖尿病胎儿进行胰岛素治疗，在导致P-丙酮酸羧激酶mRNA消失之前，会减少核转录本减少，但会增加成熟细胞质酶mRNA的丰度。对胰岛素缺乏的胎儿进行Bt2cAMP处理会产生累加效应，不仅增加成熟的P-丙酮酸羧激酶RNA转录本的丰度，还会增加大尺寸的转录本丰度。这些结果最好解释为胰岛素既抑制影响P-丙酮酸羧激酶RNA的转录，又加速转录后过程。
