Şeker Şükran, Lalegül-Ülker Özge, Elçin Ayşe Eser, Elçin Yaşar Murat
Ankara University, Ankara, Turkey.
Methods Mol Biol. 2025;2939:221-230. doi: 10.1007/7651_2024_533.
Volumetric muscle loss (VML) is one of the major types of soft tissue injury frequently encountered worldwide. In case of VML, the endogenous regenerative capacity of the skeletal muscle tissue is usually not sufficient for complete healing of the damaged area resulting in permanent functional musculoskeletal impairment. Therefore, the development of new tissue engineering approaches that will enable functional skeletal muscle regeneration by overcoming the limitations of current clinical treatments for VML injuries has become a critical goal. Platelet-rich plasma (PRP) is an inexpensive and relatively effective blood product with a high concentration of platelets containing various growth factors and cytokines involved in wound healing and tissue regeneration. Due to its autologous nature, PRP has been a safe and widely used treatment option for various wound types for many years. Recently, PRP-based biomaterials have emerged as a promising approach to promote muscle tissue regeneration upon injury. This chapter describes the use of PRP-derived fibrin microbeads as a versatile encapsulation matrix for the localized delivery of mesenchymal stem cells and growth factors to treat VML using tissue engineering strategies.
体积性肌肉损失（VML）是全球范围内常见的主要软组织损伤类型之一。在VML情况下，骨骼肌组织的内源性再生能力通常不足以使受损区域完全愈合，从而导致永久性的肌肉骨骼功能障碍。因此，开发新的组织工程方法，通过克服当前VML损伤临床治疗的局限性来实现功能性骨骼肌再生，已成为一个关键目标。富血小板血浆（PRP）是一种廉价且相对有效的血液制品，含有高浓度的血小板，其中包含参与伤口愈合和组织再生的各种生长因子和细胞因子。由于其自体性质，多年来PRP一直是治疗各种伤口类型的安全且广泛使用的治疗选择。最近，基于PRP的生物材料已成为一种有前途的方法，可促进损伤后肌肉组织的再生。本章描述了使用PRP衍生的纤维蛋白微珠作为一种通用的封装基质，通过组织工程策略将间充质干细胞和生长因子局部递送来治疗VML。
