Department of Orthopedic Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Microb Drug Resist. 2024 May;30(5):196-202. doi: 10.1089/mdr.2023.0066. Epub 2024 Apr 5.
, the principal causative agent of osteomyelitis, can be internalized by osteoblasts and thereby escape from immune phagocytes and many kinds of antibiotics. To deliver antibiotics into osteoblasts to kill in the intracellular environment, we developed gentamicin-loaded chitosan nanoparticles and evaluated their intracellular bactericidal effect. We found decreased numbers of cells in infected osteoblasts treated with gentamicin-loaded chitosan nanoparticles. The cytotoxicity of the nanoparticles was evaluated by CCK-8 assay. There was no significant viability decrease at all tested concentrations. In conclusion, our results provide evidence for the potential use of gentamicin-loaded chitosan nanoparticles to enhance the delivery of gentamicin into cells and for their antibacterial effect against internalized in the intracellular environment of osteoblasts.
金黄色葡萄球菌是骨髓炎的主要病原体，它可以被成骨细胞内化，从而逃避免疫吞噬细胞和许多种抗生素的作用。为了将抗生素递送入成骨细胞以杀死细胞内的金黄色葡萄球菌，我们开发了载庆大霉素壳聚糖纳米颗粒，并评估了它们的细胞内杀菌效果。我们发现，在用载庆大霉素壳聚糖纳米颗粒处理的感染的成骨细胞中，金黄色葡萄球菌细胞的数量减少。通过 CCK-8 法评估了纳米颗粒的细胞毒性，在所有测试浓度下均没有明显的细胞活力下降。总之，我们的结果为载庆大霉素壳聚糖纳米颗粒增强庆大霉素向细胞内的递送以及对成骨细胞内内化的金黄色葡萄球菌的抗菌作用提供了证据。
