Department of Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
BMC Microbiol. 2024 Sep 7;24(1):328. doi: 10.1186/s12866-024-03446-7.
Staphylococcus aureus is an infectious bacterium that is frequently found in healthcare settings and the community. This study aimed to prepare rutin-loaded chitosan nanoparticles (Rut-CS NPs) and assess their antibacterial activity against pathogenic strains of S. aureus.
The synthesized Rut-CS NPs exhibited an amorphous morphology with a size ranging from 160 to 240 nm and a zeta potential of 37.3 mV. Rut-CS NPs demonstrated significant antibacterial activity against S. aureus strains. Following exposure to Rut-CS NPs, the production of staphyloxanthin pigment decreased by 43.31-89.63%, leading to increased susceptibility of S. aureus to hydrogen peroxide. Additionally, visual inspection of cell morphology indicated changes in membrane integrity and permeability upon Rut-CS NPs exposure, leading to a substantial increase (107.07-191.08%) in cytoplasmic DNA leakage in the strains. Furthermore, ½ MIC of Rut-CS NPs effectively inhibited the biofilm formation (22.5-37.5%) and hemolytic activity (69-82.59%) in the S. aureus strains.
Our study showcases that Rut-CS NPs can serve as a novel treatment agent to combat S. aureus infections by altering cell morphology and inhibiting virulence factors of S. aureus.
金黄色葡萄球菌是一种常见的感染性细菌，存在于医疗保健环境和社区中。本研究旨在制备载芦丁壳聚糖纳米粒（Rut-CS NPs），并评估其对金黄色葡萄球菌致病株的抗菌活性。
合成的 Rut-CS NPs 呈无定形形态，粒径为 160-240nm，zeta 电位为 37.3mV。Rut-CS NPs 对金黄色葡萄球菌菌株表现出显著的抗菌活性。暴露于 Rut-CS NPs 后，金黄色葡萄球菌色素产量下降了 43.31-89.63%，使其对过氧化氢的敏感性增加。此外，细胞形态的直观观察表明，细胞膜完整性和通透性在 Rut-CS NPs 暴露后发生变化，导致菌株细胞质 DNA 泄漏增加 107.07-191.08%。此外，Rut-CS NPs 的 ½ MIC 有效抑制了金黄色葡萄球菌菌株的生物膜形成（22.5-37.5%）和溶血活性（69-82.59%）。
我们的研究表明，Rut-CS NPs 可以通过改变细胞形态和抑制金黄色葡萄球菌的毒力因子，成为一种新型的治疗金黄色葡萄球菌感染的药物。
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