Jiangsu Key Laboratory of Environmental Science and engineering, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Jiangsu Key Laboratory of Environmental Science and engineering, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Food Chem. 2024 Aug 15;449:139231. doi: 10.1016/j.foodchem.2024.139231. Epub 2024 Apr 2.
Pyrethroids are widely used insecticides worldwide, while their on-site and rapid detection still faces technological challenges. Herein, an innovative detection mechanism was designed for deltamethrin, a typical kind of type II pyrethroids, based on a dual-emitting fluoroprobe consisting of NH-SiQDs and Eu. Deltamethrin can rapidly hydrolyze into 3-phenoxybenzaldehyde (3-PBD) and react specifically with fluoroprobe, causing fluorescence quenching of SiQDs while maintaining the fluorescent stability of Eu. Building upon the above fluorescence-responsive principle, SiQDs@Eu provided satisfactorily dual-emitting signals, realizing the highly-selective and sensitive detection of deltamethrin. Correlation between the surface structure of SiQDs and their absorption spectra was in-depth unraveled by TD-DFT calculation and FT-IR analysis. As for the analytical performance, the recovery and LOD of deltamethrin in lettuce, provided by SiQDs@Eu, were comparable or even superior over conventional chromatographic analysis. Meanwhile, an innovative smartphone-based optical device was developed, which greatly decreased errors caused by the previously reported smartphone-based fluorescence detection.
拟除虫菊酯在全球范围内被广泛使用，但其现场和快速检测仍然面临技术挑战。在此，我们设计了一种基于由 NH-SiQDs 和 Eu 组成的双发射荧光探针的新型检测机制，用于检测典型的 II 型拟除虫菊酯-氯氟氰菊酯。氯氟氰菊酯可以迅速水解生成 3-苯氧基苯甲醛（3-PBD），并与荧光探针特异性反应，导致 SiQDs 的荧光猝灭，同时保持 Eu 的荧光稳定性。基于上述荧光响应原理，SiQDs@Eu 提供了令人满意的双发射信号，实现了对氯氟氰菊酯的高选择性和灵敏检测。通过 TD-DFT 计算和 FT-IR 分析深入揭示了 SiQDs 的表面结构与其吸收光谱之间的关系。就分析性能而言，SiQDs@Eu 提供的氯氟氰菊酯在生菜中的回收率和检出限与传统的色谱分析相当，甚至更优。同时，还开发了一种基于智能手机的创新光学设备，大大减少了之前报道的基于智能手机的荧光检测中存在的误差。
