Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; Engineering Technology Research Center of Saline-Alkaline Water Fisheries (Harbin), Chinese Academy of Fishery Sciences, Harbin 150070, China.
Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2024 Jul;281:109908. doi: 10.1016/j.cbpc.2024.109908. Epub 2024 Apr 3.
In recent years, saline-alkaline aquaculture development has become an important measure for China to expand its fishery development space to ensure food safety. Previous studies have verified that salinity and alkalinity positively influence the quality of Chinese mitten crabs (Eriocheir sinensis). However, the regulatory mechanism of E. sinensis endures saline-alkaline stress which remains obscure. This study investigated the metabolic changes in puberty-molting E. sinensis gills exposed to freshwater (FW), sodium chloride salinity of 5 ppt (SW), and carbonate alkalinity 10.00 mmol/L (AW) for 50 days using untargeted liquid chromatography-mass spectrometry metabolomics (LC-MS). A total of 5802 (positive-ion mode) and 6520 (negative-ion mode) peaks were extracted by LC-MS, respectively. A total of 188 (50 upregulated and 138 downregulated), 141 (94 upregulated and 47 downregulated), and 130 (87 upregulated and 43 downregulated) significantly regulated metabolites (SRMs) were observed in the FW-SW, FW-AW, and SW-AW treatments, respectively, wherein 42 generic SRMs were also found by Venn diagram analysis. Seven of the top 10 SRMs with the highest (variable importance in projection) VIP values were similarly identified in FW-SW and SW-AW. Integrated analysis of key metabolic pathways revealed glycerophospholipid, choline in cancer, phenylalanine, and butanoate metabolism. Overall, significant differences were observed in the metabolites and key metabolic pathways of E. sinensis gill exposed to salinity and alkalinity stress. These results will be helpful in understanding the environmental adaptability of aquatic crustaceans to saline-alkaline water.
近年来，盐碱性水产养殖的发展已成为中国拓展渔业发展空间、确保食品安全的重要举措。先前的研究已经验证了盐度和碱度会积极影响中华绒螯蟹（Eriocheir sinensis）的品质。然而，中华绒螯蟹对盐碱性胁迫的调控机制仍不清楚。本研究通过非靶向液相色谱-质谱代谢组学（LC-MS），研究了处于青春期蜕皮期的中华绒螯蟹鳃在淡水（FW）、5 ppt 氯化钠盐度（SW）和 10.00 mmol/L 碳酸盐碱度（AW）中暴露 50 天后的代谢变化。LC-MS 分别提取了 5802 个（正离子模式）和 6520 个（负离子模式）峰。FW-SW、FW-AW 和 SW-AW 处理中分别观察到 188 个（上调 50 个，下调 138 个）、141 个（上调 94 个，下调 47 个）和 130 个（上调 87 个，下调 43 个）显著调节代谢物（SRMs），通过 Venn 图分析还发现了 42 个通用 SRMs。FW-SW 和 SW-AW 中前 10 个具有最高（投影变量重要性）VIP 值的 SRMs 中有 7 个相似。关键代谢途径的综合分析揭示了甘油磷脂、癌症中的胆碱、苯丙氨酸和丁酸盐代谢。总体而言，盐度和碱度胁迫下中华绒螯蟹鳃的代谢物和关键代谢途径存在显著差异。这些结果将有助于了解水生甲壳类动物对盐碱性水的环境适应性。
