成年β-珠蛋白而非胚胎γ-珠蛋白基因在小鼠卵黄囊细胞与红白血病细胞杂交体中的选择性表达。
Selective expression of adult beta-globin but not embryonic y-globin genes in hybrids of mouse yolk-sac and erythroleukemia cells.
作者信息
Obinata M, Uematsu M, Koyama H
出版信息
Differentiation. 1985;28(3):250-3. doi: 10.1111/j.1432-0436.1985.tb00832.x.
Abstract
Hybrid cells from mouse erythroleukemia and yolk-sac cells from 12-day-old mouse embryos were formed, and the expression of adult beta-globin and embryonic y-globin genes was examined. In these hybrid cells, hemoglobin synthesis could be induced by the addition of dimethylsulfoxide. The adult beta-globin genes from both parental chromosomes were expressed, but embryonic y-globin genes were not. These results suggest that the expression of adult genes dominates that of embryonic genes.
摘要
从小鼠红白血病细胞与12日龄小鼠胚胎的卵黄囊细胞中形成了杂交细胞，并检测了成人β-珠蛋白和胚胎γ-珠蛋白基因的表达。在这些杂交细胞中，添加二甲基亚砜可诱导血红蛋白合成。来自双亲染色体的成人β-珠蛋白基因均有表达，但胚胎γ-珠蛋白基因未表达。这些结果表明成人基因的表达主导胚胎基因的表达。
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