Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Jiangxi Provincial Key Laboratory of Animal-Origin and Vector-Borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang 330038, Jiangxi, China.
Biomed Environ Sci. 2024 Mar 20;37(3):266-277. doi: 10.3967/bes2024.030.
The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area, namely, Qunlu Practice Base, Peach Blossom Garden, and Huangtong Animal Husbandry, and whether vectors carry any bacterial pathogens that may cause diseases to humans, to provide scientific basis for prospective pathogen discovery and disease prevention and control.
Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit (OPU) analysis, we characterized the species-level microbial community structure of two important vector species, biting midges and ticks, including 33 arthropod samples comprising 3,885 individuals, collected around Poyang Lake.
A total of 662 OPUs were classified in biting midges, including 195 known species and 373 potentially new species, and 618 OPUs were classified in ticks, including 217 known species and 326 potentially new species. Surprisingly, OPUs with potentially pathogenicity were detected in both arthropod vectors, with 66 known species of biting midges reported to carry potential pathogens, including and , compared to 50 in ticks, such as and . We found that was the most dominant group in both midges and ticks. Furthermore, the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria. sp7 was predominant in biting midges, while sp1 was enriched in ticks. Meanwhile, spp., which may be essential for the survival of Neumann, were detected in all tick samples. The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors.
Biting midges and ticks carry large numbers of known and potentially novel bacteria, and carry a wide range of potentially pathogenic bacteria, which may pose a risk of infection to humans and animals. The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.
本研究旨在调查鄱阳湖地区群鹿实践基地、桃花园和黄通畜牧场三个地点采集的吸血蠓和蜱的细菌群落，以及这些载体是否携带任何可能导致人类疾病的细菌病原体，为潜在病原体的发现和疾病的预防和控制提供科学依据。
本研究采用宏基因组学方法结合全长 16S rRNA 基因测序和操作分类单元（OPU）分析，对鄱阳湖地区三个地点采集的 33 个节肢动物样本（共 3885 只个体）中的两种重要的媒介物种，即吸血蠓和蜱，进行了物种水平的微生物群落结构特征分析。
在吸血蠓中共鉴定出 662 个 OPUs，包括 195 个已知种和 373 个潜在新种；在蜱中共鉴定出 618 个 OPUs，包括 217 个已知种和 326 个潜在新种。令人惊讶的是，在这两种节肢动物载体中都检测到了具有潜在致病性的 OPUs，其中报道有 66 种已知的吸血蠓携带潜在的病原体，包括 和 ，而蜱中则有 50 种，如 和 。我们发现 在蠓和蜱中都是最主要的菌群。此外，研究结果表明，蠓和蜱的微生物群往往受少数高度丰富的细菌控制。在吸血蠓中， sp7 占优势，而在蜱中， sp1 则丰富。同时，在所有蜱样本中都检测到了可能对 Neumann 生存至关重要的 spp.。本研究对吸血蠓和蜱的优势种和病原体的鉴定，拓宽了我们对节肢动物载体微生物的认识。
吸血蠓和蜱携带大量已知和潜在的新型细菌，并携带广泛的潜在致病细菌，这可能对人类和动物的感染构成威胁。蠓和蜱的微生物群落往往由少数高度丰富的细菌主导。
