Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China; Southwest United Graduate School, Kunming, 650500, China; Yunnan Key Laboratory of Panax Notoginseng, Kunming, 650500, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China; Yunnan Key Laboratory of Panax Notoginseng, Kunming, 650500, China.
J Ethnopharmacol. 2024 Aug 10;330:118148. doi: 10.1016/j.jep.2024.118148. Epub 2024 Apr 6.
The traditional Chinese herb Panax notoginseng (PN) tonifies blood, and its main active ingredient is saponin. PN is processed by different methods, resulting in different compositions and effects.
To investigate changes in the microstructure and composition of fresh PN processed by different techniques and the anti-anemia effects on tumor-bearing BALB/c mice after chemotherapy with cyclophosphamide (CTX).
Fresh PN was processed by hot-air drying (raw PN, RPN), steamed at 120 °C for 5 h (steamed PN, SPN), or fried at 130 °C, 160 °C, or 200 °C for 8 min (fried PN, FPN1, FPN2, or FPN3, respectively); then, the microstructures were compared with 3D optical microscopy, quasi-targeted metabolites were detected by liquid chromatography tandem mass spectrometry (LC‒MS/MS), and saponins were detected by high-performance liquid chromatography (HPLC). An anemic mouse model was established by subcutaneous H22 cell injection and treatment with CTX. The antianemia effects of PN after processing via three methods were investigated by measuring peripheral blood parameters, performing HE staining and measuring cell proliferation via immunofluorescence.
3D optical profiling revealed that the surface roughness of the SPN and FPN was greater than that of the other materials. Quasi-targeted metabolomics revealed that SPN and FPN had more differentially abundant metabolites whose abundance increased, while SPN had greater amounts of terpenoids and flavones. Analysis of the composition and content of the targeted saponins revealed that the contents of rare saponins (ginsenoside Rh, 20(S)-Rg, 20(R)-Rg, Rh, Rk, Rg) were greater in the SPN. In animal experiments, the RBC, WBC, HGB and HCT levels in peripheral blood were increased by SPN and FPN. HE staining and immunofluorescence showed that H-SPN and M-FPN promoted bone marrow and spleen cell proliferation.
The microstructure and components of fresh PN differed after processing via different methods. SPN and FPN ameliorated CTX-induced anemia in mice, but the effects of PN processed by these two methods did not differ.
传统中药三七具有补血作用，其主要活性成分为皂苷。三七经过不同的方法炮制，其成分和功效也有所不同。
研究不同炮制方法对鲜三七的微观结构和成分的影响，以及对环磷酰胺（CTX）化疗后荷瘤 BALB/c 小鼠的抗贫血作用。
将鲜三七分别进行热空气干燥（生三七，RPN）、120°C 蒸 5 小时（蒸制三七，SPN）或 130°C、160°C 或 200°C 油炸 8 分钟（分别为油炸三七 FPN1、FPN2 或 FPN3）；然后，用三维光学显微镜比较微观结构，用液相色谱串联质谱（LC-MS/MS）检测准靶向代谢物，用高效液相色谱（HPLC）检测皂苷。通过皮下 H22 细胞注射和环磷酰胺（CTX）处理建立贫血小鼠模型。通过测量外周血参数、进行 HE 染色和通过免疫荧光测量细胞增殖，研究三种方法处理后的三七的抗贫血作用。
三维光学轮廓显示，SPN 和 FPN 的表面粗糙度大于其他材料。准靶向代谢组学显示，SPN 和 FPN 具有更多差异丰度的代谢物，其丰度增加，而 SPN 具有更多的萜类和黄酮类化合物。对靶向皂苷的组成和含量进行分析发现，SPN 中罕见皂苷（人参皂苷 Rh、20(S)-Rg、20(R)-Rg、Rh、Rk、Rg）的含量较高。在动物实验中，SPN 和 FPN 可增加外周血 RBC、WBC、HGB 和 HCT 水平。HE 染色和免疫荧光显示，H-SPN 和 M-FPN 促进骨髓和脾脏细胞增殖。
不同炮制方法对鲜三七的微观结构和成分有不同的影响。SPN 和 FPN 改善了 CTX 诱导的小鼠贫血，但两种方法炮制的三七的效果没有差异。
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