State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China.
University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
J Agric Food Chem. 2020 Apr 29;68(17):4865-4875. doi: 10.1021/acs.jafc.0c00746. Epub 2020 Apr 20.
Saponins of (Burk.) F.H. Chen have been classified as a type of composition in functional foods for numerous diseases. However, its mild effects and other characteristics limited clinical applications in diseases. Inspired by "nine steaming and nine processing" of in traditional Chinese medicine, we developed a "steaming"-mimic protocol, which significantly changed the composition of saponins of from the original, R1, Rg1, Re, Rb1, and Rd (raw-PNS), to the products after steaming, 20/-Rh1, Rk3, Rh4, 20/-Rg3, Rk1, and Rg5 (N-PNS). Surprisingly, N-PNS demonstrated promising activities in improving hyperlipidemia and reducing body weight and weight of white adipose tissue and the inhibition of adipogenesis in obese mice. In accordance with the results , N-PNS remarkably blunted adipogenesis at the early stage of differentiation dose-dependently . Moreover, we demonstrated that the activity may involve the adenosine monophosphate (AMP)-activated protein kinase (AMPK) signaling pathway by promoting phosphorylation of AMPK and downregulating its downstream factors: sterol regulatory element binding protein 1c, stearoyl-CoA desaturase 1, and fatty acid synthase. Taken together, the steaming-induced eight compositions of saponins showed a very promising function in improving hyperlipidemia and obesity both and , providing fundamental evidence for future study and application in treatment of hyperlipidemia, obesity, and other lipid-related metabolic syndromes.
（柏科）陈的总皂甙已被归类为具有多种疾病功能食品的组成成分。然而，其温和的效果和其他特性限制了其在疾病中的临床应用。受中药“九蒸九制”的启发，我们开发了一种“蒸制”模拟方案，该方案显著改变了（柏科）总皂甙的组成，从原始的 R1、Rg1、Re、Rb1 和 Rd（生-PNS）变为蒸制后的产物 20/-Rh1、Rk3、Rh4、20/-Rg3、Rk1 和 Rg5（N-PNS）。令人惊讶的是，N-PNS 对改善高脂血症、减轻体重和白色脂肪组织重量以及抑制肥胖小鼠脂肪生成具有显著的活性。根据结果，N-PNS 显著地在分化的早期阶段通过剂量依赖性地抑制脂肪生成。此外，我们证明该活性可能涉及腺苷一磷酸（AMP）激活的蛋白激酶（AMPK）信号通路，通过促进 AMPK 的磷酸化和下调其下游因子：固醇调节元件结合蛋白 1c、硬脂酰辅酶 A 去饱和酶 1 和脂肪酸合酶。总之，蒸制诱导的八种皂甙成分在改善高脂血症和肥胖方面表现出非常有前景的功能，为今后在高脂血症、肥胖症和其他与脂质相关的代谢综合征的治疗中的研究和应用提供了基础证据。
